Detailed information NTG0684319

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2A29_RS13480
• Genome accession: NZ_CP096812
• Coordinates: 2882138..2883256 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 51248
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2877138..2888256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2A29_RS13470 (M2A29_13465)	-	2880254..2880946 (-)	693	WP_272409671.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2A29_RS13475 (M2A29_13470)	pilT	2881073..2882110 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M2A29_RS13480 (M2A29_13475)	pilU	2882138..2883256 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M2A29_RS13485 (M2A29_13480)	fur	2883326..2883763 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M2A29_RS13490 (M2A29_13485)	bamE	2883875..2884273 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M2A29_RS13495 (M2A29_13490)	-	2884328..2884651 (-)	324	WP_000442589.1	RnfH family protein	-
M2A29_RS13500 (M2A29_13495)	-	2884651..2885718 (-)	1068	WP_118978229.1	hypothetical protein	-
M2A29_RS13505 (M2A29_13500)	-	2885871..2886326 (-)	456	WP_000782976.1	bacteriohemerythrin	-
M2A29_RS13510 (M2A29_13505)	-	2886579..2887427 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=684319 M2A29_RS13480 WP_000347036.1 2882138..2883256(+) (pilU) [Acinetobacter baumannii strain 51248]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=684319 M2A29_RS13480 WP_000347036.1 2882138..2883256(+) (pilU) [Acinetobacter baumannii strain 51248]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392