Detailed information NTG0684133

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA40_RS02495
• Genome accession: NZ_CP110349
• Coordinates: 544581..545729 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA40
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 539581..550729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA40_RS02470	-	540732..541028 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA40_RS02475 (PALA40_00489)	-	541025..541618 (-)	594	WP_003084544.1	YggT family protein	-
PALA40_RS02480 (PALA40_00490)	proC	541629..542450 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PALA40_RS02485 (PALA40_00491)	-	542462..543154 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA40_RS02490 (PALA40_00492)	pilT	543369..544403 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA40_RS02495 (PALA40_00493)	pilU	544581..545729 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA40_RS02500 (PALA40_00494)	czcD	545737..546636 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA40_RS02505 (PALA40_00495)	-	546791..547195 (+)	405	WP_003084560.1	NINE protein	-
PALA40_RS02510 (PALA40_00496)	-	547438..548811 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
PALA40_RS02515 (PALA40_00497)	-	548808..549992 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=684133 PALA40_RS02495 WP_003100959.1 544581..545729(+) (pilU) [Pseudomonas aeruginosa strain PALA40]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=684133 PALA40_RS02495 WP_003100959.1 544581..545729(+) (pilU) [Pseudomonas aeruginosa strain PALA40]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382