Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OFY13_RS02345 Genome accession   NZ_CP110188
Coordinates   486091..486576 (-) Length   161 a.a.
NCBI ID   WP_000649332.1    Uniprot ID   -
Organism   Vibrio cholerae strain E1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481091..491576
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OFY13_RS02325 (OFY13_02325) coaE 481596..482204 (-) 609 WP_000011556.1 dephospho-CoA kinase -
  OFY13_RS02330 (OFY13_02330) pilD 482201..483076 (-) 876 WP_000418751.1 A24 family peptidase Machinery gene
  OFY13_RS02335 (OFY13_02335) pilC 483134..484360 (-) 1227 WP_000648513.1 type II secretion system F family protein Machinery gene
  OFY13_RS02340 (OFY13_02340) pilB 484403..486091 (-) 1689 WP_000957192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OFY13_RS02345 (OFY13_02345) pilA 486091..486576 (-) 486 WP_000649332.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OFY13_RS02350 (OFY13_02350) nadC 486821..487711 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OFY13_RS02355 (OFY13_02355) ampD 488009..488554 (+) 546 WP_000567319.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OFY13_RS02360 (OFY13_02360) fldB 488705..489223 (-) 519 WP_000690112.1 flavodoxin FldB -
  OFY13_RS02365 (OFY13_02365) xerD 489361..490269 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  OFY13_RS02370 (OFY13_02370) dsbC 490286..491038 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16458.81 Da        Isoelectric Point: 8.8070

>NTDB_id=683401 OFY13_RS02345 WP_000649332.1 486091..486576(-) (pilA) [Vibrio cholerae strain E1]
MKAYKNKQQQGFTLIELMIVVAIIGILAAFAVPAYQNYTNKTHASELLNASSAIKAGVGVCLLSGYSDCKQGANNVPATQ
TFDKGSSDKFAITSGVEVTSPATSSAAATITGAVVATVDATAGKAGLKKSGTLTLTPKLTTSGVTWTITCDLGSQDYCPK
G

Nucleotide


Download         Length: 486 bp        

>NTDB_id=683401 OFY13_RS02345 WP_000649332.1 486091..486576(-) (pilA) [Vibrio cholerae strain E1]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGCATTTT
GGCTGCTTTTGCTGTACCAGCATACCAAAACTATACAAATAAAACTCATGCATCAGAATTACTCAATGCATCATCAGCAA
TTAAAGCTGGTGTAGGTGTGTGTCTTTTAAGTGGCTACTCTGATTGCAAACAAGGTGCTAACAATGTCCCAGCTACACAA
ACTTTTGATAAAGGAAGTTCGGATAAGTTTGCAATTACTTCAGGTGTAGAAGTCACAAGCCCTGCAACGTCGAGCGCAGC
AGCAACAATCACTGGAGCTGTCGTTGCGACAGTGGATGCAACTGCTGGCAAAGCTGGTTTGAAGAAATCCGGCACATTAA
CTTTAACACCCAAATTAACCACATCAGGAGTCACGTGGACTATCACATGTGATCTAGGCTCACAAGATTACTGTCCAAAA
GGCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

38.235

100

0.404
  pilA Haemophilus influenzae 86-028NP

40.69

90.062

0.366
  pilA2 Legionella pneumophila str. Paris

39.456

91.304

0.36
  comP Acinetobacter baylyi ADP1

37.908

95.031

0.36