Detailed information NTG0682508

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M1S54_RS15405
• Genome accession: NZ_CP096690
• Coordinates: 3143562..3144680 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 5839
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3138562..3149680

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1S54_RS15395 (M1S54_15395)	-	3141678..3142370 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
M1S54_RS15400 (M1S54_15400)	pilT	3142497..3143534 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
M1S54_RS15405 (M1S54_15405)	pilU	3143562..3144680 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M1S54_RS15410 (M1S54_15410)	fur	3144750..3145187 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
M1S54_RS15415 (M1S54_15415)	bamE	3145299..3145697 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
M1S54_RS15420 (M1S54_15420)	-	3145752..3146075 (-)	324	WP_000442587.1	RnfH family protein	-
M1S54_RS15425 (M1S54_15425)	-	3146075..3147142 (-)	1068	WP_002019572.1	hypothetical protein	-
M1S54_RS15430 (M1S54_15430)	-	3147295..3147750 (-)	456	WP_000782976.1	bacteriohemerythrin	-
M1S54_RS15435 (M1S54_15435)	-	3148003..3148851 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=682508 M1S54_RS15405 WP_000347039.1 3143562..3144680(+) (pilU) [Acinetobacter baumannii strain 5839]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=682508 M1S54_RS15405 WP_000347039.1 3143562..3144680(+) (pilU) [Acinetobacter baumannii strain 5839]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395