Detailed information NTG0680784

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA51_RS02275
• Genome accession: NZ_CP109851
• Coordinates: 467926..469074 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA51
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 462926..474074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA51_RS02250	-	464077..464373 (-)	297	WP_031655578.1	DUF167 domain-containing protein	-
PALA51_RS02255 (PALA51_00446)	-	464370..464963 (-)	594	WP_003084544.1	YggT family protein	-
PALA51_RS02260 (PALA51_00447)	proC	464974..465795 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA51_RS02265 (PALA51_00448)	-	465807..466499 (-)	693	WP_003117895.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA51_RS02270 (PALA51_00449)	pilT	466714..467748 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA51_RS02275 (PALA51_00450)	pilU	467926..469074 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA51_RS02280 (PALA51_00451)	czcD	469082..469981 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA51_RS02285 (PALA51_00452)	-	470135..470539 (+)	405	WP_003084560.1	NINE protein	-
PALA51_RS02290 (PALA51_00453)	-	470782..472155 (+)	1374	WP_003117896.1	pyridoxal-phosphate dependent enzyme	-
PALA51_RS02295 (PALA51_00454)	-	472152..473336 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=680784 PALA51_RS02275 WP_003100959.1 467926..469074(+) (pilU) [Pseudomonas aeruginosa strain PALA51]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=680784 PALA51_RS02275 WP_003100959.1 467926..469074(+) (pilU) [Pseudomonas aeruginosa strain PALA51]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382