Detailed information NTG0680474

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA33_RS02415
• Genome accession: NZ_CP109845
• Coordinates: 532936..534084 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA33
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 527936..539084

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA33_RS02390	-	529087..529383 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA33_RS02395 (PALA33_00471)	-	529380..529973 (-)	594	WP_003084544.1	YggT family protein	-
PALA33_RS02400 (PALA33_00472)	proC	529984..530805 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PALA33_RS02405 (PALA33_00473)	-	530817..531509 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA33_RS02410 (PALA33_00474)	pilT	531724..532758 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA33_RS02415 (PALA33_00475)	pilU	532936..534084 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA33_RS02420 (PALA33_00476)	czcD	534092..534991 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA33_RS02425 (PALA33_00477)	-	535145..535549 (+)	405	WP_003084560.1	NINE protein	-
PALA33_RS02430 (PALA33_00478)	-	535792..537165 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
PALA33_RS02435 (PALA33_00479)	-	537162..538346 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=680474 PALA33_RS02415 WP_003100959.1 532936..534084(+) (pilU) [Pseudomonas aeruginosa strain PALA33]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=680474 PALA33_RS02415 WP_003100959.1 532936..534084(+) (pilU) [Pseudomonas aeruginosa strain PALA33]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382