Detailed information NTG0680388

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M0D10_RS23830
• Genome accession: NZ_CP096207
• Coordinates: 5080674..5081822 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa TBCF10839
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5075674..5086822

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M0D10_RS23810 (M0D10_23755)	-	5076412..5077596 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
M0D10_RS23815 (M0D10_23760)	-	5077593..5078966 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M0D10_RS23820 (M0D10_23765)	-	5079209..5079613 (-)	405	WP_003084560.1	NINE protein	-
M0D10_RS23825 (M0D10_23770)	czcD	5079767..5080666 (+)	900	WP_042853545.1	CDF family zinc transporter CzcD	-
M0D10_RS23830 (M0D10_23775)	pilU	5080674..5081822 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M0D10_RS23835 (M0D10_23780)	pilT	5082000..5083034 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M0D10_RS23840 (M0D10_23785)	-	5083249..5083941 (+)	693	WP_023464234.1	YggS family pyridoxal phosphate-dependent enzyme	-
M0D10_RS23845 (M0D10_23790)	proC	5083953..5084774 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M0D10_RS23850 (M0D10_23795)	-	5084785..5085378 (+)	594	WP_003084544.1	YggT family protein	-
M0D10_RS23855 (M0D10_23800)	-	5085375..5085671 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=680388 M0D10_RS23830 WP_003100959.1 5080674..5081822(-) (pilU) [Pseudomonas aeruginosa TBCF10839]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=680388 M0D10_RS23830 WP_003100959.1 5080674..5081822(-) (pilU) [Pseudomonas aeruginosa TBCF10839]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382