Detailed information NTG0680271

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA29_RS02030
• Genome accession: NZ_CP109843
• Coordinates: 436510..437658 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA29
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431510..442658

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA29_RS02005	-	432661..432957 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA29_RS02010 (PALA29_00397)	-	432954..433547 (-)	594	WP_003084544.1	YggT family protein	-
PALA29_RS02015 (PALA29_00398)	proC	433558..434379 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA29_RS02020 (PALA29_00399)	-	434391..435083 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA29_RS02025 (PALA29_00400)	pilT	435298..436332 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA29_RS02030 (PALA29_00401)	pilU	436510..437658 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA29_RS02035 (PALA29_00402)	czcD	437666..438565 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA29_RS02040 (PALA29_00403)	-	438719..439123 (+)	405	WP_003084560.1	NINE protein	-
PALA29_RS02045 (PALA29_00404)	-	439366..440739 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA29_RS02050 (PALA29_00405)	-	440736..441920 (+)	1185	WP_023114752.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=680271 PALA29_RS02030 WP_003100959.1 436510..437658(+) (pilU) [Pseudomonas aeruginosa strain PALA29]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=680271 PALA29_RS02030 WP_003100959.1 436510..437658(+) (pilU) [Pseudomonas aeruginosa strain PALA29]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382