Detailed information NTG0679996

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA25_RS02045
• Genome accession: NZ_CP109834
• Coordinates: 438613..439761 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433613..444761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA25_RS02020	-	434764..435060 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA25_RS02025 (PALA25_00406)	-	435057..435650 (-)	594	WP_003084544.1	YggT family protein	-
PALA25_RS02030 (PALA25_00407)	proC	435661..436482 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA25_RS02035 (PALA25_00408)	-	436494..437186 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA25_RS02040 (PALA25_00409)	pilT	437401..438435 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA25_RS02045 (PALA25_00410)	pilU	438613..439761 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA25_RS02050 (PALA25_00411)	czcD	439769..440668 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA25_RS02055 (PALA25_00412)	-	440823..441227 (+)	405	WP_003084560.1	NINE protein	-
PALA25_RS02060 (PALA25_00413)	-	441470..442843 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA25_RS02065 (PALA25_00414)	-	442840..444024 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=679996 PALA25_RS02045 WP_003100959.1 438613..439761(+) (pilU) [Pseudomonas aeruginosa strain PALA25]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=679996 PALA25_RS02045 WP_003100959.1 438613..439761(+) (pilU) [Pseudomonas aeruginosa strain PALA25]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382