Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MY524_RS13545 Genome accession   NZ_CP096017
Coordinates   2969421..2970542 (+) Length   373 a.a.
NCBI ID   WP_250656413.1    Uniprot ID   -
Organism   Alkalimarinus coralli strain SCSIO 12638     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2964421..2975542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY524_RS13525 - 2965698..2966285 (-) 588 WP_250656410.1 YggT family protein -
  MY524_RS13530 proC 2966525..2967355 (-) 831 WP_250656411.1 pyrroline-5-carboxylate reductase -
  MY524_RS13535 - 2967415..2968182 (-) 768 WP_338021695.1 YggS family pyridoxal phosphate-dependent enzyme -
  MY524_RS13540 pilT 2968324..2969358 (+) 1035 WP_250656412.1 type IV pilus twitching motility protein PilT Machinery gene
  MY524_RS13545 pilU 2969421..2970542 (+) 1122 WP_250656413.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MY524_RS13550 polA 2970672..2973416 (-) 2745 WP_250656414.1 DNA polymerase I -
  MY524_RS13555 - 2973477..2973776 (+) 300 WP_250656415.1 DUF2782 domain-containing protein -
  MY524_RS13560 - 2973893..2974888 (+) 996 WP_250656416.1 homoserine kinase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41314.45 Da        Isoelectric Point: 6.2834

>NTDB_id=679171 MY524_RS13545 WP_250656413.1 2969421..2970542(+) (pilU) [Alkalimarinus coralli strain SCSIO 12638]
MDFDKLLKVMVEKGGSDLFITAGVPPSIKIHGKIVPVTKSSLTPEQTREIVLNTMSEKQQTEFMDNHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPKVDDLGLPEIIKTLSMTKRGLIIFVGATGTGKSTSLASMIGHRNRNSQGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVIMIGEIRSKETMDYAVTFAETGHLCLATLHANNANQALDRI
INFFPPEHHNQIWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINSPLCADMIRKSEVHKLKELMTKSRDIGMQTFDQA
LYDLYSAGEITYEDALAYADSANDLRLMIKLGADSGGDKLSSSLDGLSISDDI

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=679171 MY524_RS13545 WP_250656413.1 2969421..2970542(+) (pilU) [Alkalimarinus coralli strain SCSIO 12638]
ATGGACTTCGATAAACTGTTAAAAGTGATGGTTGAGAAAGGTGGATCGGATCTTTTTATTACCGCTGGGGTTCCGCCAAG
TATCAAGATTCACGGCAAGATCGTACCGGTGACTAAATCCTCCTTAACGCCTGAGCAAACTCGCGAAATCGTGTTGAACA
CGATGAGTGAGAAGCAGCAGACTGAGTTTATGGATAACCATGAGTGCAATTTTGCGATCAGTGCGCGTGGTATCGGACGA
TTTCGGGTCAGCGCATTCTATCAGCGTAACCTCTGCGGCATGGTGCTGCGTCGTATTGAGACCAATATACCTAAGGTTGA
CGACCTGGGCTTGCCGGAAATCATTAAAACCCTGTCAATGACCAAGCGCGGGCTCATTATTTTCGTGGGGGCAACAGGTA
CAGGTAAGTCTACCTCTCTCGCGTCGATGATTGGTCATCGCAACCGCAATAGTCAGGGGCATATTATTTCGATTGAGGAC
CCGATTGAGTACATTCATCAGCATCAGGGGTGTATTGTAACTCAGCGCGAAGTGGGGATTGATACCGAAAGCTTTGATGT
TGCCCTGAAAAATACGCTACGTCAGGCGCCCGACGTCATTATGATTGGTGAGATCCGCTCGAAGGAGACCATGGATTATG
CTGTGACGTTCGCAGAAACGGGCCACCTCTGCTTAGCAACCTTGCACGCAAACAACGCGAACCAGGCGCTTGATAGAATT
ATCAACTTCTTCCCGCCGGAGCACCACAACCAGATATGGATGGATCTCTCGCTGAACCTTAAAGCGATTGTTGCTCAACA
GTTGATCCCAACCCCTGATGGTAAGGGCAGAAGGGCGGTCATCGAGGTGCTGATTAACTCGCCGCTCTGCGCTGATATGA
TTCGCAAGTCAGAAGTGCATAAACTCAAGGAGTTGATGACGAAGTCGCGGGATATTGGTATGCAAACCTTTGATCAGGCA
TTGTATGACCTCTACTCAGCGGGTGAAATCACCTACGAGGATGCATTGGCCTATGCGGATTCGGCTAACGACTTGAGACT
GATGATCAAGCTTGGTGCAGATTCTGGAGGGGACAAGCTCTCATCTTCGCTTGATGGCCTCTCGATCAGCGATGATATTT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.738

100

0.769
  pilU Acinetobacter baylyi ADP1

64.706

95.71

0.619
  pilU Vibrio cholerae strain A1552

54.201

98.928

0.536
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.385

97.587

0.394