Detailed information NTG0679098

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MX621_RS02180
• Genome accession: NZ_CP095923
• Coordinates: 475792..476940 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain AR19438
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 470792..481940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MX621_RS02155 (MX621_02155)	-	471943..472239 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MX621_RS02160 (MX621_02160)	-	472236..472829 (-)	594	WP_003084544.1	YggT family protein	-
MX621_RS02165 (MX621_02165)	proC	472840..473661 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MX621_RS02170 (MX621_02170)	-	473673..474365 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MX621_RS02175 (MX621_02175)	pilT	474580..475614 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MX621_RS02180 (MX621_02180)	pilU	475792..476940 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
MX621_RS02185 (MX621_02185)	czcD	476948..477847 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MX621_RS02190 (MX621_02190)	-	478001..478405 (+)	405	WP_003084560.1	NINE protein	-
MX621_RS02195 (MX621_02195)	-	478648..480021 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
MX621_RS02200 (MX621_02200)	-	480018..481202 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=679098 MX621_RS02180 WP_003110016.1 475792..476940(+) (pilU) [Pseudomonas aeruginosa strain AR19438]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=679098 MX621_RS02180 WP_003110016.1 475792..476940(+) (pilU) [Pseudomonas aeruginosa strain AR19438]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382