Detailed information NTG0679065

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MX649_RS02065
• Genome accession: NZ_CP095922
• Coordinates: 441216..442364 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain AR19583
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 436216..447364

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MX649_RS02040 (MX649_02040)	-	437367..437663 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
MX649_RS02045 (MX649_02045)	-	437660..438253 (-)	594	WP_003084544.1	YggT family protein	-
MX649_RS02050 (MX649_02050)	proC	438264..439085 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MX649_RS02055 (MX649_02055)	-	439097..439789 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MX649_RS02060 (MX649_02060)	pilT	440004..441038 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MX649_RS02065 (MX649_02065)	pilU	441216..442364 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MX649_RS02070 (MX649_02070)	czcD	442372..443271 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MX649_RS02075 (MX649_02075)	-	443425..443829 (+)	405	WP_003084560.1	NINE protein	-
MX649_RS02080 (MX649_02080)	-	444072..445445 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
MX649_RS02085 (MX649_02085)	-	445442..446626 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=679065 MX649_RS02065 WP_003100959.1 441216..442364(+) (pilU) [Pseudomonas aeruginosa strain AR19583]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=679065 MX649_RS02065 WP_003100959.1 441216..442364(+) (pilU) [Pseudomonas aeruginosa strain AR19583]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382