Detailed information NTG0679032

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MX651_RS02085
• Genome accession: NZ_CP095920
• Coordinates: 443844..444992 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain AR19640
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 438844..449992

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MX651_RS02060 (MX651_02060)	-	439995..440291 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
MX651_RS02065 (MX651_02065)	-	440288..440881 (-)	594	WP_003084544.1	YggT family protein	-
MX651_RS02070 (MX651_02070)	proC	440892..441713 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MX651_RS02075 (MX651_02075)	-	441725..442417 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MX651_RS02080 (MX651_02080)	pilT	442632..443666 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MX651_RS02085 (MX651_02085)	pilU	443844..444992 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MX651_RS02090 (MX651_02090)	czcD	445000..445899 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MX651_RS02095 (MX651_02095)	-	446053..446457 (+)	405	WP_003084560.1	NINE protein	-
MX651_RS02100 (MX651_02100)	-	446700..448073 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
MX651_RS02105 (MX651_02105)	-	448070..449254 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=679032 MX651_RS02085 WP_003100959.1 443844..444992(+) (pilU) [Pseudomonas aeruginosa strain AR19640]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=679032 MX651_RS02085 WP_003100959.1 443844..444992(+) (pilU) [Pseudomonas aeruginosa strain AR19640]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382