Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y97_RS16615 Genome accession   NZ_CP095742
Coordinates   3694938..3696101 (-) Length   387 a.a.
NCBI ID   WP_247428760.1    Uniprot ID   -
Organism   Luteibacter aegosomatissinici strain 353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3689938..3701101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y97_RS16585 (L2Y97_16585) - 3690002..3690331 (-) 330 WP_247428748.1 hypothetical protein -
  L2Y97_RS16590 (L2Y97_16590) - 3690423..3690989 (+) 567 WP_247428750.1 YqgE/AlgH family protein -
  L2Y97_RS16595 (L2Y97_16595) ruvX 3690993..3691412 (+) 420 WP_247428752.1 Holliday junction resolvase RuvX -
  L2Y97_RS16600 (L2Y97_16600) - 3691448..3692374 (+) 927 WP_247428754.1 aspartate carbamoyltransferase catalytic subunit -
  L2Y97_RS16605 (L2Y97_16605) - 3692451..3694199 (+) 1749 WP_247428756.1 hypothetical protein -
  L2Y97_RS16610 (L2Y97_16610) - 3694488..3694853 (+) 366 WP_247428758.1 BON domain-containing protein -
  L2Y97_RS16615 (L2Y97_16615) pilU 3694938..3696101 (-) 1164 WP_247428760.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y97_RS16620 (L2Y97_16620) pilT 3696238..3697275 (-) 1038 WP_247428762.1 type IV pilus twitching motility protein PilT Machinery gene
  L2Y97_RS16625 (L2Y97_16625) proC 3697393..3698217 (+) 825 WP_247428764.1 pyrroline-5-carboxylate reductase -
  L2Y97_RS16630 (L2Y97_16630) - 3698214..3698789 (+) 576 WP_247428766.1 YggT family protein -
  L2Y97_RS16635 (L2Y97_16635) - 3698793..3700916 (-) 2124 WP_247428768.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43336.62 Da        Isoelectric Point: 6.9972

>NTDB_id=678004 L2Y97_RS16615 WP_247428760.1 3694938..3696101(-) (pilU) [Luteibacter aegosomatissinici strain 353]
MSEFDFTSFLKLMVHKQASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPAQREEFEKTHECQFAISASG
VGRFRVSCFYQRNCVGMVLRRIESKIPTPEELNLPPIIKQLAMTKRGIIIFVGGTGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHRHEGCIITQRELGIDTDNWENALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRILHFFPEDRRSALFMDLSLNLKGIVAQQLIPTPDGKARRVAIEVLLGTPLAQDYIRQGEIHKLKELMRDSNQLGMRTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVEIDKANDKNTFGGMLHR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=678004 L2Y97_RS16615 WP_247428760.1 3694938..3696101(-) (pilU) [Luteibacter aegosomatissinici strain 353]
ATGAGCGAGTTCGATTTCACCTCCTTTCTTAAGCTGATGGTGCACAAGCAGGCGTCCGACCTGTTCATCACCGCCGGCGT
CGCACCCTCCATGAAGGTGCAGGGCCGTATCGTGCCCATCACGCAAAGCCCCCTCTCCGCGCAGCAATCGCGCGACATGG
TGCTAAACGTGATGACGCCGGCCCAGCGCGAGGAATTTGAAAAAACCCACGAGTGCCAGTTCGCGATCAGCGCCTCGGGC
GTCGGCCGCTTCCGTGTCTCGTGCTTTTACCAGCGCAACTGCGTGGGCATGGTCCTGCGCCGTATCGAATCAAAGATTCC
CACGCCGGAAGAGCTGAACCTGCCGCCGATCATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTGGGTG
GTACCGGTACCGGTAAATCCACGTCGCTCGCCTCGATGATCGGCTACCGCAACCAGAATTCCACGGGTCACATCATCACG
ATCGAGGACCCGATCGAATACGTGCATCGCCATGAAGGCTGCATCATCACCCAGCGCGAGCTCGGTATCGATACCGATAA
CTGGGAAAACGCGCTGAAGAACACGCTGCGCCAGGCGCCAGATGTCATCATGATCGGCGAAGTGCGTACGCGTGAGACGA
TGGAGCACGCCATCAACTTTTCGGAAACGGGCCACCTGTGCCTTTGCACGCTGCATGCGAACAATGCCAACCAGGCGCTG
GACCGCATCCTGCACTTCTTCCCGGAAGACCGTCGCAGCGCGCTGTTCATGGATCTGTCCCTGAACCTGAAGGGCATCGT
GGCGCAGCAGCTCATTCCCACGCCCGACGGTAAGGCACGTCGCGTGGCGATCGAAGTGCTGCTGGGTACGCCGCTCGCGC
AGGACTACATCCGTCAGGGCGAGATCCACAAGCTCAAGGAGCTCATGCGCGATTCCAACCAGCTTGGCATGCGCACGTTC
GACCAGAGCCTGGTGGAGCTCTACCACGCGGGCGAAATCTCGTACGAAGATGCCCTGCGCCATGCCGACAGCTCCAACGA
AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCACACGCTGTCGCAGGGCCTGGAAGGCGTTGAGATCGACA
AGGCCAACGACAAGAACACGTTCGGCGGCATGCTGCACCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.775

96.641

0.636
  pilU Acinetobacter baylyi ADP1

62.921

91.99

0.579
  pilU Vibrio cholerae strain A1552

51.081

95.607

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.667

96.899

0.375