Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NALG_RS01905 Genome accession   NZ_AP017373
Coordinates   401881..403137 (+) Length   418 a.a.
NCBI ID   WP_096368377.1    Uniprot ID   -
Organism   Lactococcus formosensis strain 122061     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396881..408137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NALG_RS01880 (NALG_0396) glyQ 397031..397999 (-) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -
  NALG_RS01885 (NALG_0397) - 398013..398792 (-) 780 WP_096368375.1 formate/nitrite transporter family protein -
  NALG_RS01890 (NALG_0398) - 399110..400131 (+) 1022 Protein_383 alpha/beta hydrolase -
  NALG_RS01895 (NALG_0400) - 400168..401190 (-) 1023 WP_019293557.1 DUF475 domain-containing protein -
  NALG_RS01900 (NALG_0401) - 401203..401826 (-) 624 WP_096368376.1 YigZ family protein -
  NALG_RS01905 (NALG_0402) comFA 401881..403137 (+) 1257 WP_096368377.1 DEAD/DEAH box helicase Machinery gene
  NALG_RS01910 (NALG_0403) - 403134..403778 (+) 645 WP_096368378.1 ComF family protein -
  NALG_RS01915 (NALG_0404) - 403781..404242 (-) 462 WP_096368379.1 diacylglycerol kinase family protein -
  NALG_RS01920 (NALG_0405) ybeY 404226..404714 (-) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  NALG_RS01925 (NALG_0406) - 404724..405197 (-) 474 WP_017369708.1 NUDIX hydrolase -
  NALG_RS01930 (NALG_0407) - 405199..406158 (-) 960 WP_096368380.1 PhoH family protein -
  NALG_RS01935 (NALG_0408) - 406277..407383 (-) 1107 WP_096368381.1 alanine dehydrogenase -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47857.06 Da        Isoelectric Point: 10.3108

>NTDB_id=67793 NALG_RS01905 WP_096368377.1 401881..403137(+) (comFA) [Lactococcus formosensis strain 122061]
MNELYGRLLLQKELTKIPDKAILFDGMKEVSKTIVMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGRVRSDEKLYHLPQKN
FAAVSCLKWQGKLTGPQQYISDNLLNLHRQQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSPRIDVCLELYYRLK
RNFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLILDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRKSGFPLLIFVPEIRLGEKLCQDLQTYFPHEEIAFVAS
TSLERLASVERFRQGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTSSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEANKNIRNMNKAGEFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=67793 NALG_RS01905 WP_096368377.1 401881..403137(+) (comFA) [Lactococcus formosensis strain 122061]
ATGAATGAATTATACGGTAGACTTCTTCTGCAAAAAGAATTGACGAAGATACCGGACAAAGCCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTGTCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCCCATTGTATTCAGATGGGGCGTGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAAAT
TTTGCTGCAGTTTCTTGTCTAAAATGGCAGGGAAAGCTCACAGGTCCTCAGCAATATATCTCCGATAATTTACTTAATCT
GCATCGGCAGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCCGCGTATTGATGTTTGTCTAGAACTTTACTATCGCTTAAAG
AGGAATTTTTCCTGTCCCATCTCTCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCCCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTGATTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTACTTTGCGCTGGCTCAAGCTCGAAAACCCTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGGCAAATCGAAAAGCTGCAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGGTCAAAAAACAACGAAAATCTGGTTTTCCATTACTTATCTTTGTCC
CTGAAATAAGACTAGGAGAAAAACTGTGCCAAGACTTACAAACCTATTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGCATCTGTGGAACGTTTTCGACAAGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCATCATAATTTCACAAGTTCAAGTCTCATTC
AAATTGCTGGACGAGTAGGACGAAGTACTGAAAGACCTGATGGTAAGGTATTTTTCTTTCATTTAGGAAAAACAACAGCG
ATGTTGGAAGCCAATAAAAACATCAGAAATATGAATAAAGCTGGAGAATTCCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(275-382)

Type III restriction enzyme, res subunit (ResIII)

(93-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.644

95.455

0.55
  comFA/cflA Streptococcus pneumoniae TIGR4

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae Rx1

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae D39

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae R6

52.358

100

0.531
  comFA/cflA Streptococcus mitis SK321

51.887

100

0.526
  comFA/cflA Streptococcus mitis NCTC 12261

50.943

100

0.517
  comFA Bacillus subtilis subsp. subtilis str. 168

37.9

100

0.397
  comFA Latilactobacillus sakei subsp. sakei 23K

38.25

95.694

0.366

Multiple sequence alignment