Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MX663_RS13910 Genome accession   NZ_CP095736
Coordinates   2648486..2649103 (-) Length   205 a.a.
NCBI ID   WP_014480312.1    Uniprot ID   -
Organism   Bacillus subtilis strain N4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2645516..2687586 2648486..2649103 within 0


Gene organization within MGE regions


Location: 2645516..2687586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX663_RS13900 (MX663_13900) comEC 2645516..2647846 (-) 2331 WP_033881047.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MX663_RS13905 (MX663_13905) comEB 2647850..2648419 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MX663_RS13910 (MX663_13910) comEA 2648486..2649103 (-) 618 WP_014480312.1 competence protein ComEA Machinery gene
  MX663_RS13915 (MX663_13915) comER 2649187..2650008 (+) 822 WP_247470579.1 late competence protein ComER -
  MX663_RS13920 (MX663_13920) yqeM 2650074..2650817 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  MX663_RS13925 (MX663_13925) rsfS 2650814..2651170 (-) 357 WP_041333759.1 ribosome silencing factor -
  MX663_RS13930 (MX663_13930) yqeK 2651188..2651748 (-) 561 WP_247470580.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MX663_RS13935 (MX663_13935) nadD 2651738..2652307 (-) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  MX663_RS13940 (MX663_13940) yhbY 2652319..2652609 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MX663_RS13945 (MX663_13945) aroE 2652603..2653445 (-) 843 WP_029317883.1 shikimate dehydrogenase -
  MX663_RS13950 (MX663_13950) yqeH 2653463..2654563 (-) 1101 WP_003229966.1 ribosome biogenesis GTPase YqeH -
  MX663_RS13955 (MX663_13955) yqeG 2654567..2655085 (-) 519 WP_003226126.1 YqeG family HAD IIIA-type phosphatase -
  MX663_RS13960 (MX663_13960) - 2655447..2655587 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MX663_RS13965 (MX663_13965) yqeF 2655893..2656624 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  MX663_RS13970 (MX663_13970) cwlH 2656876..2657628 (-) 753 WP_247470581.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MX663_RS13975 (MX663_13975) yqeD 2657815..2658441 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  MX663_RS13980 (MX663_13980) gnd 2658460..2659353 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MX663_RS13985 (MX663_13985) yqeB 2659605..2660327 (+) 723 WP_010886572.1 hypothetical protein -
  MX663_RS13990 (MX663_13990) nucA/comI 2660360..2660770 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MX663_RS13995 (MX663_13995) - 2660966..2661313 (+) 348 Protein_2716 sigma-70 family RNA polymerase sigma factor -
  MX663_RS14000 (MX663_14000) spoIVCA 2661344..2662804 (-) 1461 WP_240304280.1 site-specific DNA recombinase SpoIVCA -
  MX663_RS14005 (MX663_14005) - 2662762..2662940 (-) 179 Protein_2718 hypothetical protein -
  MX663_RS14010 (MX663_14010) - 2663597..2664190 (-) 594 WP_080528950.1 TetR/AcrR family transcriptional regulator -
  MX663_RS14015 (MX663_14015) - 2664349..2665344 (+) 996 WP_088299191.1 acryloyl-CoA reductase -
  MX663_RS14020 (MX663_14020) - 2665626..2665756 (+) 131 Protein_2721 DUF255 domain-containing protein -
  MX663_RS14025 (MX663_14025) - 2666157..2667170 (-) 1014 WP_247470582.1 NADP-dependent oxidoreductase -
  MX663_RS14030 (MX663_14030) - 2667437..2668186 (-) 750 WP_247470584.1 SDR family NAD(P)-dependent oxidoreductase -
  MX663_RS14035 (MX663_14035) - 2668553..2669338 (-) 786 WP_247470585.1 SDR family oxidoreductase -
  MX663_RS14040 (MX663_14040) - 2669674..2670732 (-) 1059 WP_141949631.1 alkene reductase -
  MX663_RS14045 (MX663_14045) - 2670747..2671595 (-) 849 WP_141949629.1 alpha/beta hydrolase -
  MX663_RS14050 (MX663_14050) - 2671706..2672281 (-) 576 WP_141949627.1 TetR/AcrR family transcriptional regulator -
  MX663_RS14055 (MX663_14055) - 2672857..2673693 (-) 837 WP_141949624.1 helix-turn-helix transcriptional regulator -
  MX663_RS14060 (MX663_14060) fabF 2673797..2675029 (+) 1233 WP_141949622.1 beta-ketoacyl-ACP synthase II -
  MX663_RS14065 (MX663_14065) - 2675201..2675443 (+) 243 WP_141949620.1 DUF255 domain-containing protein -
  MX663_RS14070 (MX663_14070) yqcK 2675440..2675879 (-) 440 Protein_2731 ArsI/CadI family heavy metal resistance metalloenzyme -
  MX663_RS14075 (MX663_14075) arsR 2675938..2676254 (-) 317 Protein_2732 arsenical resistance operon transcriptional regulator ArsR -
  MX663_RS14080 (MX663_14080) rapE 2676673..2677800 (+) 1128 WP_141949616.1 response regulator aspartate phosphatase RapE -
  MX663_RS14085 (MX663_14085) phrE 2677790..2677924 (+) 135 WP_014114495.1 phosphatase RapE inhibitor PhrE -
  MX663_RS14090 (MX663_14090) - 2678153..2679925 (+) 1773 WP_141949614.1 T7SS effector LXG polymorphic toxin -
  MX663_RS14095 (MX663_14095) - 2679938..2680399 (+) 462 WP_029318109.1 SMI1/KNR4 family protein -
  MX663_RS14100 (MX663_14100) - 2680605..2681327 (-) 723 WP_015251658.1 NPP1 family protein -
  MX663_RS14105 (MX663_14105) - 2681521..2682486 (-) 966 Protein_2738 N-acetylmuramoyl-L-alanine amidase -
  MX663_RS14110 (MX663_14110) - 2682532..2682672 (-) 141 Protein_2739 phage holin family protein -
  MX663_RS14115 (MX663_14115) - 2682699..2682884 (-) 186 Protein_2740 phage terminase large subunit -
  MX663_RS14120 (MX663_14120) terS 2682877..2683672 (-) 796 Protein_2741 phage terminase small subunit -
  MX663_RS14125 (MX663_14125) - 2683854..2683940 (+) 87 WP_073991391.1 YjcZ family sporulation protein -
  MX663_RS14130 (MX663_14130) - 2683981..2684073 (+) 93 WP_088326573.1 YjcZ family sporulation protein -
  MX663_RS14135 (MX663_14135) cotD 2684259..2684474 (-) 216 WP_076458314.1 spore coat protein CotD -
  MX663_RS14140 (MX663_14140) yqaQ 2685033..2685442 (-) 410 Protein_2745 sigma factor-like helix-turn-helix DNA-binding protein -
  MX663_RS14145 (MX663_14145) - 2685740..2686006 (+) 267 WP_029318115.1 hypothetical protein -
  MX663_RS14150 (MX663_14150) - 2686111..2686294 (-) 184 Protein_2747 hypothetical protein -
  MX663_RS14155 (MX663_14155) yqaO 2686366..2686572 (-) 207 Protein_2748 XtrA/YqaO family protein -
  MX663_RS14160 (MX663_14160) yqaN 2686656..2687075 (-) 420 Protein_2749 RusA family crossover junction endodeoxyribonuclease -
  MX663_RS14165 (MX663_14165) - 2687171..2687320 (-) 150 WP_076458162.1 hypothetical protein -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21781.51 Da        Isoelectric Point: 4.7220

>NTDB_id=677876 MX663_RS13910 WP_014480312.1 2648486..2649103(-) (comEA) [Bacillus subtilis strain N4]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=677876 MX663_RS13910 WP_014480312.1 2648486..2649103(-) (comEA) [Bacillus subtilis strain N4]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.512

100

0.995
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39