Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | MX663_RS13990 | Genome accession | NZ_CP095736 |
| Coordinates | 2660360..2660770 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain N4 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 2645516..2687586 | 2660360..2660770 | within | 0 |
Gene organization within MGE regions
Location: 2645516..2687586
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MX663_RS13900 (MX663_13900) | comEC | 2645516..2647846 (-) | 2331 | WP_033881047.1 | DNA internalization-related competence protein ComEC/Rec2 | Machinery gene |
| MX663_RS13905 (MX663_13905) | comEB | 2647850..2648419 (-) | 570 | WP_003229978.1 | ComE operon protein 2 | - |
| MX663_RS13910 (MX663_13910) | comEA | 2648486..2649103 (-) | 618 | WP_014480312.1 | competence protein ComEA | Machinery gene |
| MX663_RS13915 (MX663_13915) | comER | 2649187..2650008 (+) | 822 | WP_247470579.1 | late competence protein ComER | - |
| MX663_RS13920 (MX663_13920) | yqeM | 2650074..2650817 (-) | 744 | WP_003229973.1 | class I SAM-dependent methyltransferase | - |
| MX663_RS13925 (MX663_13925) | rsfS | 2650814..2651170 (-) | 357 | WP_041333759.1 | ribosome silencing factor | - |
| MX663_RS13930 (MX663_13930) | yqeK | 2651188..2651748 (-) | 561 | WP_247470580.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK | - |
| MX663_RS13935 (MX663_13935) | nadD | 2651738..2652307 (-) | 570 | WP_041333756.1 | nicotinate-nucleotide adenylyltransferase | - |
| MX663_RS13940 (MX663_13940) | yhbY | 2652319..2652609 (-) | 291 | WP_003226133.1 | ribosome assembly RNA-binding protein YhbY | - |
| MX663_RS13945 (MX663_13945) | aroE | 2652603..2653445 (-) | 843 | WP_029317883.1 | shikimate dehydrogenase | - |
| MX663_RS13950 (MX663_13950) | yqeH | 2653463..2654563 (-) | 1101 | WP_003229966.1 | ribosome biogenesis GTPase YqeH | - |
| MX663_RS13955 (MX663_13955) | yqeG | 2654567..2655085 (-) | 519 | WP_003226126.1 | YqeG family HAD IIIA-type phosphatase | - |
| MX663_RS13960 (MX663_13960) | - | 2655447..2655587 (+) | 141 | WP_003226124.1 | sporulation histidine kinase inhibitor Sda | - |
| MX663_RS13965 (MX663_13965) | yqeF | 2655893..2656624 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| MX663_RS13970 (MX663_13970) | cwlH | 2656876..2657628 (-) | 753 | WP_247470581.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| MX663_RS13975 (MX663_13975) | yqeD | 2657815..2658441 (+) | 627 | WP_038427798.1 | TVP38/TMEM64 family protein | - |
| MX663_RS13980 (MX663_13980) | gnd | 2658460..2659353 (-) | 894 | WP_043857850.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| MX663_RS13985 (MX663_13985) | yqeB | 2659605..2660327 (+) | 723 | WP_010886572.1 | hypothetical protein | - |
| MX663_RS13990 (MX663_13990) | nucA/comI | 2660360..2660770 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| MX663_RS13995 (MX663_13995) | - | 2660966..2661313 (+) | 348 | Protein_2716 | sigma-70 family RNA polymerase sigma factor | - |
| MX663_RS14000 (MX663_14000) | spoIVCA | 2661344..2662804 (-) | 1461 | WP_240304280.1 | site-specific DNA recombinase SpoIVCA | - |
| MX663_RS14005 (MX663_14005) | - | 2662762..2662940 (-) | 179 | Protein_2718 | hypothetical protein | - |
| MX663_RS14010 (MX663_14010) | - | 2663597..2664190 (-) | 594 | WP_080528950.1 | TetR/AcrR family transcriptional regulator | - |
| MX663_RS14015 (MX663_14015) | - | 2664349..2665344 (+) | 996 | WP_088299191.1 | acryloyl-CoA reductase | - |
| MX663_RS14020 (MX663_14020) | - | 2665626..2665756 (+) | 131 | Protein_2721 | DUF255 domain-containing protein | - |
| MX663_RS14025 (MX663_14025) | - | 2666157..2667170 (-) | 1014 | WP_247470582.1 | NADP-dependent oxidoreductase | - |
| MX663_RS14030 (MX663_14030) | - | 2667437..2668186 (-) | 750 | WP_247470584.1 | SDR family NAD(P)-dependent oxidoreductase | - |
| MX663_RS14035 (MX663_14035) | - | 2668553..2669338 (-) | 786 | WP_247470585.1 | SDR family oxidoreductase | - |
| MX663_RS14040 (MX663_14040) | - | 2669674..2670732 (-) | 1059 | WP_141949631.1 | alkene reductase | - |
| MX663_RS14045 (MX663_14045) | - | 2670747..2671595 (-) | 849 | WP_141949629.1 | alpha/beta hydrolase | - |
| MX663_RS14050 (MX663_14050) | - | 2671706..2672281 (-) | 576 | WP_141949627.1 | TetR/AcrR family transcriptional regulator | - |
| MX663_RS14055 (MX663_14055) | - | 2672857..2673693 (-) | 837 | WP_141949624.1 | helix-turn-helix transcriptional regulator | - |
| MX663_RS14060 (MX663_14060) | fabF | 2673797..2675029 (+) | 1233 | WP_141949622.1 | beta-ketoacyl-ACP synthase II | - |
| MX663_RS14065 (MX663_14065) | - | 2675201..2675443 (+) | 243 | WP_141949620.1 | DUF255 domain-containing protein | - |
| MX663_RS14070 (MX663_14070) | yqcK | 2675440..2675879 (-) | 440 | Protein_2731 | ArsI/CadI family heavy metal resistance metalloenzyme | - |
| MX663_RS14075 (MX663_14075) | arsR | 2675938..2676254 (-) | 317 | Protein_2732 | arsenical resistance operon transcriptional regulator ArsR | - |
| MX663_RS14080 (MX663_14080) | rapE | 2676673..2677800 (+) | 1128 | WP_141949616.1 | response regulator aspartate phosphatase RapE | - |
| MX663_RS14085 (MX663_14085) | phrE | 2677790..2677924 (+) | 135 | WP_014114495.1 | phosphatase RapE inhibitor PhrE | - |
| MX663_RS14090 (MX663_14090) | - | 2678153..2679925 (+) | 1773 | WP_141949614.1 | T7SS effector LXG polymorphic toxin | - |
| MX663_RS14095 (MX663_14095) | - | 2679938..2680399 (+) | 462 | WP_029318109.1 | SMI1/KNR4 family protein | - |
| MX663_RS14100 (MX663_14100) | - | 2680605..2681327 (-) | 723 | WP_015251658.1 | NPP1 family protein | - |
| MX663_RS14105 (MX663_14105) | - | 2681521..2682486 (-) | 966 | Protein_2738 | N-acetylmuramoyl-L-alanine amidase | - |
| MX663_RS14110 (MX663_14110) | - | 2682532..2682672 (-) | 141 | Protein_2739 | phage holin family protein | - |
| MX663_RS14115 (MX663_14115) | - | 2682699..2682884 (-) | 186 | Protein_2740 | phage terminase large subunit | - |
| MX663_RS14120 (MX663_14120) | terS | 2682877..2683672 (-) | 796 | Protein_2741 | phage terminase small subunit | - |
| MX663_RS14125 (MX663_14125) | - | 2683854..2683940 (+) | 87 | WP_073991391.1 | YjcZ family sporulation protein | - |
| MX663_RS14130 (MX663_14130) | - | 2683981..2684073 (+) | 93 | WP_088326573.1 | YjcZ family sporulation protein | - |
| MX663_RS14135 (MX663_14135) | cotD | 2684259..2684474 (-) | 216 | WP_076458314.1 | spore coat protein CotD | - |
| MX663_RS14140 (MX663_14140) | yqaQ | 2685033..2685442 (-) | 410 | Protein_2745 | sigma factor-like helix-turn-helix DNA-binding protein | - |
| MX663_RS14145 (MX663_14145) | - | 2685740..2686006 (+) | 267 | WP_029318115.1 | hypothetical protein | - |
| MX663_RS14150 (MX663_14150) | - | 2686111..2686294 (-) | 184 | Protein_2747 | hypothetical protein | - |
| MX663_RS14155 (MX663_14155) | yqaO | 2686366..2686572 (-) | 207 | Protein_2748 | XtrA/YqaO family protein | - |
| MX663_RS14160 (MX663_14160) | yqaN | 2686656..2687075 (-) | 420 | Protein_2749 | RusA family crossover junction endodeoxyribonuclease | - |
| MX663_RS14165 (MX663_14165) | - | 2687171..2687320 (-) | 150 | WP_076458162.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=677877 MX663_RS13990 WP_009967785.1 2660360..2660770(-) (nucA/comI) [Bacillus subtilis strain N4]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=677877 MX663_RS13990 WP_009967785.1 2660360..2660770(-) (nucA/comI) [Bacillus subtilis strain N4]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |