Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   MU520_RS08015 Genome accession   NZ_CP095463
Coordinates   1636392..1637057 (-) Length   221 a.a.
NCBI ID   WP_004194119.1    Uniprot ID   A0A140EWV9
Organism   Streptococcus suis strain TJS56     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1635751..1700489 1636392..1637057 within 0


Gene organization within MGE regions


Location: 1635751..1700489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU520_RS08010 raiA 1635773..1636315 (-) 543 WP_004194117.1 ribosome-associated translation inhibitor RaiA -
  MU520_RS08015 comFC/cflB 1636392..1637057 (-) 666 WP_004194119.1 ComF family protein Machinery gene
  MU520_RS08020 comFA/cflA 1637050..1638342 (-) 1293 WP_012774986.1 DEAD/DEAH box helicase Machinery gene
  MU520_RS08025 - 1638399..1639031 (+) 633 WP_014636235.1 YigZ family protein -
  MU520_RS08030 cysK 1639121..1640047 (+) 927 WP_012774985.1 cysteine synthase A -
  MU520_RS08035 - 1640073..1640444 (-) 372 WP_011921997.1 S1 RNA-binding domain-containing protein -
  MU520_RS08040 - 1640446..1641846 (-) 1401 WP_011921996.1 Cof-type HAD-IIB family hydrolase -
  MU520_RS08045 - 1641896..1642537 (-) 642 WP_002937025.1 response regulator transcription factor -
  MU520_RS08050 - 1642509..1643525 (-) 1017 WP_002937028.1 sensor histidine kinase -
  MU520_RS08055 liaF 1643522..1644181 (-) 660 WP_223375661.1 cell wall-active antibiotics response protein LiaF -
  MU520_RS08060 stkP 1644638..1646632 (-) 1995 WP_011921992.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  MU520_RS08065 - 1646632..1647369 (-) 738 WP_012774983.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  MU520_RS08070 rsmB 1647407..1648720 (-) 1314 WP_011921990.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  MU520_RS08075 fmt 1648707..1649645 (-) 939 WP_011921989.1 methionyl-tRNA formyltransferase -
  MU520_RS08080 - 1649777..1652167 (-) 2391 WP_012774982.1 primosomal protein N' -
  MU520_RS08085 rpoZ 1652360..1652674 (-) 315 WP_004194153.1 DNA-directed RNA polymerase subunit omega -
  MU520_RS08090 gmk 1652698..1653324 (-) 627 WP_012774981.1 guanylate kinase -
  MU520_RS08095 - 1653479..1655092 (-) 1614 WP_011921986.1 ribonuclease Y -
  MU520_RS08100 - 1655222..1655704 (+) 483 WP_004194159.1 S-ribosylhomocysteine lyase -
  MU520_RS08105 - 1656087..1657541 (-) 1455 WP_024409455.1 cell division site-positioning protein MapZ family protein -
  MU520_RS08110 - 1657551..1658717 (-) 1167 WP_011921983.1 class I SAM-dependent RNA methyltransferase -
  MU520_RS08120 gpsB 1659274..1659609 (-) 336 WP_004194167.1 cell division regulator GpsB -
  MU520_RS08125 - 1659680..1660219 (-) 540 WP_004194171.1 DUF1273 domain-containing protein -
  MU520_RS08130 recU 1660285..1660902 (+) 618 WP_004194173.1 Holliday junction resolvase RecU -
  MU520_RS08135 pbp1a 1660883..1663057 (+) 2175 WP_014636231.1 penicillin-binding protein PBP1A -
  MU520_RS08140 rsmG 1663116..1663829 (+) 714 WP_011921980.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  MU520_RS08145 - 1663871..1664074 (-) 204 WP_004195223.1 cold-shock protein -
  MU520_RS08150 - 1664234..1665067 (-) 834 WP_011921979.1 energy-coupling factor transporter transmembrane component T -
  MU520_RS08155 - 1665064..1666737 (-) 1674 WP_011921978.1 ABC transporter ATP-binding protein -
  MU520_RS08160 - 1666855..1667097 (-) 243 WP_011921977.1 lactoylglutathione lyase -
  MU520_RS08165 - 1667424..1668407 (-) 984 WP_009909407.1 rhodanese-related sulfurtransferase -
  MU520_RS08170 - 1668620..1669540 (-) 921 WP_012774980.1 hypothetical protein -
  MU520_RS08175 - 1669648..1670193 (-) 546 WP_011921974.1 ECF-type riboflavin transporter substrate-binding protein -
  MU520_RS08180 - 1670214..1671062 (-) 849 WP_004194192.1 S-adenosyl-l-methionine hydroxide adenosyltransferase family protein -
  MU520_RS08185 - 1671572..1672789 (-) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -
  MU520_RS08190 - 1672871..1673074 (-) 204 WP_000814511.1 excisionase -
  MU520_RS08195 - 1673058..1673309 (+) 252 WP_001845478.1 hypothetical protein -
  MU520_RS08200 - 1673535..1673765 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  MU520_RS08205 - 1673762..1674184 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  MU520_RS08210 - 1674689..1675042 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  MU520_RS08215 - 1675102..1675269 (-) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  MU520_RS08220 tet(M) 1675388..1677307 (-) 1920 WP_000691736.1 tetracycline resistance ribosomal protection protein Tet(M) -
  MU520_RS08225 - 1677323..1677439 (-) 117 WP_001814923.1 tetracycline resistance determinant leader peptide -
  MU520_RS08230 - 1677684..1678616 (-) 933 WP_001224318.1 conjugal transfer protein -
  MU520_RS08235 - 1678613..1679614 (-) 1002 WP_000769868.1 bifunctional lysozyme/C40 family peptidase -
  MU520_RS08240 - 1679611..1681788 (-) 2178 WP_000804748.1 membrane protein -
  MU520_RS08245 - 1681791..1684238 (-) 2448 WP_000331160.1 ATP-binding protein -
  MU520_RS08250 - 1684222..1684614 (-) 393 WP_000723888.1 conjugal transfer protein -
  MU520_RS08255 - 1684703..1685200 (-) 498 WP_000342539.1 antirestriction protein ArdA -
  MU520_RS08260 - 1685317..1685538 (-) 222 WP_001009056.1 hypothetical protein -
  MU520_RS08265 mobT 1685581..1686786 (-) 1206 WP_000398284.1 MobT family relaxase -
  MU520_RS08270 - 1686809..1686961 (-) 153 WP_000879507.1 hypothetical protein -
  MU520_RS08275 - 1686964..1688349 (-) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  MU520_RS08280 - 1688378..1688764 (-) 387 WP_000985015.1 YdcP family protein -
  MU520_RS08285 - 1688780..1689094 (-) 315 WP_000420682.1 YdcP family protein -
  MU520_RS08290 - 1689423..1690139 (-) 717 WP_004194195.1 YebC/PmpR family DNA-binding transcriptional regulator -
  MU520_RS08295 - 1690300..1690611 (+) 312 WP_004194196.1 PadR family transcriptional regulator -
  MU520_RS08300 - 1690622..1691248 (+) 627 WP_011922666.1 DUF2812 domain-containing protein -
  MU520_RS08305 - 1691319..1693487 (-) 2169 WP_011922665.1 PTS transporter subunit IIBC -
  MU520_RS08310 - 1693521..1694312 (-) 792 WP_024409456.1 endonuclease/exonuclease/phosphatase family protein -
  MU520_RS08315 - 1694511..1695218 (+) 708 WP_004194205.1 UTRA domain-containing protein -
  MU520_RS08320 glgP 1695446..1697710 (-) 2265 WP_012774979.1 glycogen/starch/alpha-glucan family phosphorylase -
  MU520_RS08325 malQ 1697703..1699211 (-) 1509 WP_011922661.1 4-alpha-glucanotransferase -

Sequence

Protein


Download         Length: 221 a.a.        Molecular weight: 25748.55 Da        Isoelectric Point: 8.2726

>NTDB_id=677015 MU520_RS08015 WP_004194119.1 1636392..1637057(-) (comFC/cflB) [Streptococcus suis strain TJS56]
MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQHCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYN
QAMAHYFSRYKFQGDYVLRNIFAKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLDATNIPYQSLLGKYESQKQS
SKNRAERLEAKQMFYLLDEKEVPEKILLFDDIYTTGATIQLAVELFMKIGRKEIKTFSLTR

Nucleotide


Download         Length: 666 bp        

>NTDB_id=677015 MU520_RS08015 WP_004194119.1 1636392..1637057(-) (comFC/cflB) [Streptococcus suis strain TJS56]
ATGTCTAATTGCCTATTGTGCGGTCAAGCTATGAAAAATAAAACAAGATTTTCAGATCTCATCTTCTTTAGTAAAGAAAA
ATCGGGCATCTGTGAAGAATGTTTTTCGACTTTTGAGGAAATAGCAGAGCAACACTGTCCACATTGTTATAAAAATGGGG
AATCGGAAAGTTGCAAGGATTGTCAGTACTGGCAGAATCAAGGGAAACCAGTTGTCCACACAGCTTTGTACCAATATAAT
CAAGCGATGGCGCATTATTTTAGTCGCTACAAATTTCAAGGAGACTATGTTTTAAGAAATATTTTTGCTAAAAAGCTTAG
AATAGCGTTAAGCCAATTTCCTGACTACACAATTGTTCCCATACCAATTAGTCAAAAACGACTATCAGAACGCGGTTTTA
ATCAAGTGGAAGGACTTTTGGATGCTACAAATATTCCCTATCAGTCCCTTCTTGGAAAGTATGAAAGCCAAAAACAATCA
TCTAAAAATCGTGCAGAGAGACTAGAAGCAAAACAGATGTTCTATCTATTAGATGAAAAAGAAGTGCCAGAAAAAATATT
ATTATTCGACGATATATATACAACAGGAGCCACAATCCAACTTGCTGTAGAACTTTTCATGAAAATTGGTAGGAAAGAAA
TCAAAACATTTTCACTAACACGATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A140EWV9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus mitis SK321

49.083

98.643

0.484
  comFC/cflB Streptococcus pneumoniae TIGR4

49.083

98.643

0.484
  comFC/cflB Streptococcus pneumoniae Rx1

49.083

98.643

0.484
  comFC/cflB Streptococcus pneumoniae D39

49.083

98.643

0.484
  comFC/cflB Streptococcus pneumoniae R6

49.083

98.643

0.484
  comFC/cflB Streptococcus mitis NCTC 12261

49.083

98.643

0.484