Detailed information NTG0067603

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IOMTU133_RS02585
• Genome accession: NZ_AP017302
• Coordinates: 572930..574078 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain IOMTU 133
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 567930..579078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IOMTU133_RS02560	-	569081..569377 (-)	297	WP_031632852.1	DUF167 domain-containing protein	-
IOMTU133_RS02565 (IOMTU133_0541)	-	569374..569967 (-)	594	WP_003084544.1	YggT family protein	-
IOMTU133_RS02570 (IOMTU133_0542)	proC	569978..570799 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
IOMTU133_RS02575 (IOMTU133_0543)	-	570811..571503 (-)	693	WP_060852980.1	YggS family pyridoxal phosphate-dependent enzyme	-
IOMTU133_RS02580 (IOMTU133_0544)	pilT	571718..572752 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IOMTU133_RS02585 (IOMTU133_0545)	pilU	572930..574078 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
IOMTU133_RS02590 (IOMTU133_0546)	czcD	574086..574985 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IOMTU133_RS02595 (IOMTU133_0547)	-	575140..575544 (+)	405	WP_003084560.1	NINE protein	-
IOMTU133_RS02600 (IOMTU133_0548)	-	575787..577160 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
IOMTU133_RS02605 (IOMTU133_0549)	-	577157..578341 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=67603 IOMTU133_RS02585 WP_003110016.1 572930..574078(+) (pilU) [Pseudomonas aeruginosa strain IOMTU 133]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=67603 IOMTU133_RS02585 WP_003110016.1 572930..574078(+) (pilU) [Pseudomonas aeruginosa strain IOMTU 133]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382