NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	MUK77_RS02200	Genome accession	NZ_CP095086
Coordinates	453422..454546 (+)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli isolate EC5654
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 448422..459546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUK77_RS02175 (MUK77_02175)	mscL	448539..448952 (-)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-
MUK77_RS02180 (MUK77_02180)	trkA	449082..450458 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
MUK77_RS02185 (MUK77_02185)	rsmB	450480..451769 (-)	1290	WP_000744773.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
MUK77_RS02190 (MUK77_02190)	fmt	451821..452768 (-)	948	WP_000004425.1	methionyl-tRNA formyltransferase	-
MUK77_RS02195 (MUK77_02195)	def	452783..453292 (-)	510	WP_000114984.1	peptide deformylase	-
MUK77_RS02200 (MUK77_02200)	dprA	453422..454546 (+)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
MUK77_RS02205 (MUK77_02205)	smg	454518..454991 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
MUK77_RS02210 (MUK77_02210)	yrdD	455020..455562 (+)	543	WP_001312137.1	type I DNA topoisomerase	-
MUK77_RS02215 (MUK77_02215)	tsaC	455567..456139 (+)	573	WP_001468260.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
MUK77_RS02220 (MUK77_02220)	aroE	456144..456962 (+)	819	WP_000451249.1	shikimate dehydrogenase	-
MUK77_RS02225 (MUK77_02225)	yrdB	456959..457216 (+)	258	WP_001070563.1	DUF1488 domain-containing protein	-
MUK77_RS02230 (MUK77_02230)	yrdA	457192..457746 (-)	555	WP_001286206.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=675148 MUK77_RS02200 WP_000228517.1 453422..454546(+) (dprA) [Escherichia coli isolate EC5654]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=675148 MUK77_RS02200 WP_000228517.1 453422..454546(+) (dprA) [Escherichia coli isolate EC5654]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGCCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0675148