Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MTZ49_RS08370 Genome accession   NZ_CP094972
Coordinates   1749021..1751207 (+) Length   728 a.a.
NCBI ID   WP_264747857.1    Uniprot ID   -
Organism   Entomomonas sp. E2T0     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1744021..1756207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTZ49_RS08350 (MTZ49_08345) pilM 1746211..1747269 (+) 1059 WP_413774138.1 type IV pilus assembly protein PilM -
  MTZ49_RS08355 (MTZ49_08350) - 1747269..1747838 (+) 570 WP_264745099.1 PilN domain-containing protein -
  MTZ49_RS08360 (MTZ49_08355) - 1747835..1748437 (+) 603 WP_264745100.1 type 4a pilus biogenesis protein PilO -
  MTZ49_RS08365 (MTZ49_08360) - 1748434..1748964 (+) 531 WP_264745101.1 pilus assembly protein PilP -
  MTZ49_RS08370 (MTZ49_08365) pilQ 1749021..1751207 (+) 2187 WP_264747857.1 type IV pilus secretin PilQ Machinery gene
  MTZ49_RS08375 (MTZ49_08370) aroK 1751212..1751727 (+) 516 WP_319804726.1 shikimate kinase AroK -
  MTZ49_RS08380 (MTZ49_08375) aroB 1751772..1752875 (+) 1104 WP_264745103.1 3-dehydroquinate synthase -
  MTZ49_RS08385 (MTZ49_08380) - 1752880..1754382 (+) 1503 WP_264745104.1 ATPase, T2SS/T4P/T4SS family -
  MTZ49_RS08390 (MTZ49_08385) queF 1754385..1755218 (+) 834 WP_264745105.1 NADPH-dependent 7-cyano-7-deazaguanine reductase QueF -
  MTZ49_RS08395 (MTZ49_08390) - 1755235..1755501 (-) 267 WP_264745106.1 DUF4404 family protein -

Sequence

Protein


Download         Length: 728 a.a.        Molecular weight: 79238.82 Da        Isoelectric Point: 5.5659

>NTDB_id=674628 MTZ49_RS08370 WP_264747857.1 1749021..1751207(+) (pilQ) [Entomomonas sp. E2T0]
MNVSLIRLSVALLGMAIPSILYAANLKDMDVVSLPGERVELKLMFDEPVAAPNGYTIDQPARIALDLPGVTSQLGEKRRA
VGIGNVRNLTVVESKNVTRLVVGLNTLVPYTTRAEGNNIFIILGDKTTASPVAQTNTASTVSTKPTNNVTPVTTKAVTSV
GNAIENIDFRRGDNGEGNIVVKLSNSTISPDVQEKGGKIVLNFPKTQLPESLRVRLDVKDFATPVQFINSTAKSDGSSIS
IEPKGNYDYLVFQANDELTVSVKHVSAEEKQRRLEDQGVFKGDTLSLNFQDIEVRSVLQILADFTKLNLVATDSVTGNIT
LRLQNVPWDQALDIIMRTRGLAKRQEGNILIIAPADELAESARRMFDAEQTLKATQPLRRELIPVNYADATSIIALFQAT
MSQTNSVQTNQNGAYNANMTSNDRGSLTVDKRTNTIIAYQTEERLEELRRIITQLDRPVRQVQIEARIVEANVDFAKSLG
VRWGADIGSKSRNWQSYGGADGRVNSEGAGKDFGNLGTSITHDGDNYMWTPAAPVVDLGAANATSGIGIGFFSSNVMIDL
KLNAMQKSGQGEIISQPKVTTSDKETARIMKGQEVPYQEASSSGATSTSFKEALLSLEVTPQITPDNQIMMAVTVTKDSV
NESRTYQGVPPINKNEVNAKVLVSDGETIVLGGVFQNEQSKVTEKVPFLGDLPVMGRLFRRDTVSNTKQELLIFITPRII
DSKAFASR

Nucleotide


Download         Length: 2187 bp        

>NTDB_id=674628 MTZ49_RS08370 WP_264747857.1 1749021..1751207(+) (pilQ) [Entomomonas sp. E2T0]
ATGAATGTTTCTTTAATACGTTTAAGTGTAGCTTTATTAGGTATGGCTATTCCCTCAATATTATATGCAGCTAATTTAAA
AGATATGGATGTTGTTTCTTTACCTGGCGAACGGGTAGAGCTTAAATTAATGTTTGATGAACCAGTAGCAGCGCCTAATG
GTTATACAATTGATCAGCCTGCACGTATTGCCCTTGATTTACCTGGTGTTACAAGCCAATTAGGTGAGAAAAGAAGGGCT
GTTGGTATAGGTAATGTAAGAAATCTAACAGTAGTTGAATCTAAAAATGTAACGCGATTAGTGGTAGGCTTAAATACTTT
AGTACCTTATACAACGCGTGCAGAAGGCAATAATATTTTTATTATATTAGGTGATAAAACTACGGCATCACCAGTTGCTC
AAACAAATACTGCTTCTACAGTATCTACTAAGCCAACAAATAATGTTACGCCAGTTACCACAAAAGCAGTTACCTCAGTT
GGAAATGCTATTGAAAATATTGATTTTAGACGTGGTGATAATGGTGAAGGTAATATTGTCGTTAAGTTATCAAATAGTAC
AATTAGTCCTGATGTACAAGAAAAGGGAGGTAAAATAGTTCTTAATTTCCCTAAAACACAATTACCTGAATCATTACGTG
TACGGTTAGATGTTAAAGATTTTGCAACGCCTGTGCAGTTTATTAACTCAACAGCTAAATCGGATGGTTCGAGTATTTCT
ATTGAGCCTAAGGGAAATTATGATTATTTAGTTTTTCAAGCTAATGATGAATTAACTGTTAGTGTTAAACATGTATCTGC
AGAAGAAAAACAACGCCGTTTAGAAGATCAAGGTGTTTTTAAAGGTGATACTTTATCTTTAAATTTCCAAGATATTGAAG
TAAGAAGTGTATTACAAATTCTAGCAGATTTTACTAAATTAAATTTAGTGGCTACTGATTCAGTAACAGGTAATATTACT
TTACGTTTACAAAATGTACCATGGGATCAAGCATTAGATATTATCATGCGTACAAGAGGGCTTGCAAAGCGTCAAGAAGG
TAATATTCTTATTATTGCTCCTGCTGATGAGTTAGCAGAGTCTGCTCGTAGAATGTTTGATGCTGAACAAACATTAAAAG
CAACACAACCTTTACGTCGTGAGCTTATCCCTGTTAATTATGCTGATGCTACAAGTATTATTGCATTATTCCAAGCAACT
ATGTCCCAGACTAATAGTGTACAAACTAATCAGAATGGTGCTTATAATGCGAATATGACTAGTAATGATCGAGGTTCTTT
GACTGTTGATAAGCGTACTAACACGATTATTGCTTATCAAACAGAAGAACGTTTAGAAGAGTTAAGACGTATTATCACTC
AGTTAGACCGACCTGTGCGTCAAGTACAAATTGAAGCACGTATTGTTGAGGCTAATGTTGATTTCGCTAAAAGCCTAGGG
GTTCGTTGGGGAGCTGATATTGGCTCAAAATCTCGTAATTGGCAAAGTTATGGTGGTGCTGATGGTCGTGTAAACTCTGA
GGGCGCTGGCAAAGATTTTGGTAATCTTGGTACATCGATTACTCATGATGGAGATAACTACATGTGGACACCAGCAGCAC
CAGTAGTAGACTTAGGTGCGGCAAATGCCACCTCAGGTATTGGTATAGGTTTTTTTAGTTCGAATGTAATGATTGATTTA
AAATTAAATGCTATGCAGAAATCTGGACAAGGTGAGATAATTTCTCAACCGAAAGTAACAACTTCAGATAAAGAAACTGC
AAGGATTATGAAAGGTCAAGAAGTACCTTATCAAGAAGCCTCTTCAAGTGGTGCTACTTCTACTTCATTTAAGGAGGCAT
TACTTTCTTTAGAGGTAACTCCACAAATTACTCCAGATAATCAAATTATGATGGCTGTTACAGTGACTAAAGACTCTGTT
AATGAGTCACGGACATATCAAGGTGTTCCACCCATTAATAAAAATGAAGTTAATGCTAAAGTATTAGTGTCTGATGGTGA
AACTATTGTATTAGGTGGTGTGTTCCAGAATGAACAAAGTAAAGTAACAGAAAAAGTACCATTCTTAGGTGATTTACCTG
TGATGGGACGCTTGTTCCGTCGTGATACTGTATCTAATACTAAACAAGAGTTATTAATTTTTATAACTCCACGTATTATT
GATAGTAAGGCGTTTGCTTCGAGATAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(49-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-461)

Bacterial type II and III secretion system protein (Secretin)

(564-721)

Secretin and TonB N terminus short domain (STN)

(308-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.422

99.863

0.593
  pilQ Acinetobacter baumannii D1279779

39.544

100

0.405
  pilQ Acinetobacter baumannii strain A118

39.544

100

0.405
  comQ Acinetobacter baylyi ADP1

40.398

96.566

0.39