Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LEN_RS19780 Genome accession   NZ_AP014940
Coordinates   4960820..4961878 (-) Length   352 a.a.
NCBI ID   WP_074864544.1    Uniprot ID   A0AAU9B378
Organism   Lysobacter enzymogenes strain M497-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4955820..4966878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEN_RS19760 (LEN_4005) pilQ 4956632..4958581 (-) 1950 WP_232518550.1 type IV pilus secretin PilQ -
  LEN_RS19765 (LEN_4006) - 4958756..4959298 (-) 543 WP_074864541.1 pilus assembly protein PilP -
  LEN_RS19770 (LEN_4007) pilO 4959298..4959996 (-) 699 WP_096380069.1 type 4a pilus biogenesis protein PilO -
  LEN_RS19775 (LEN_4008) - 4959993..4960820 (-) 828 WP_096380074.1 PilN domain-containing protein -
  LEN_RS19780 (LEN_4009) pilM 4960820..4961878 (-) 1059 WP_074864544.1 pilus assembly protein PilM Machinery gene
  LEN_RS19785 (LEN_4010) - 4962478..4965027 (+) 2550 WP_096380078.1 penicillin-binding protein 1A -
  LEN_RS19790 (LEN_4011) - 4965431..4966084 (+) 654 WP_096380081.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37701.03 Da        Isoelectric Point: 4.6373

>NTDB_id=67460 LEN_RS19780 WP_074864544.1 4960820..4961878(-) (pilM) [Lysobacter enzymogenes strain M497-1]
MGLITKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRAKHAAAA
VAGSSVITRVIGMPADLDEDDLESQVELEAANYVPYPIDEVNHDFEVLGPMPGSPDMVQVLLAASRSENVEVRASALELG
GLTPKVMDVEAFAIENAFALLADTLSSPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGIPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=67460 LEN_RS19780 WP_074864544.1 4960820..4961878(-) (pilM) [Lysobacter enzymogenes strain M497-1]
GTGGGACTCATCACAAAAAGCCAGCCGGCTCTCGTCGGCGTGGATATCAGTTCGACCGCGGTAAAGTTGCTGCAACTTTC
GCGTGCGGGCAATCGCTACCGCGTTGAGCATTACGCGGTCGAGCCGTTGCCGCCGAACGCGGTGGTGGAAAAGAACATCG
TCGAGGTCGAAGCGGTGGGCGAGGCGATCAAACGCGCCGTCGCCCGCTCGGGGACCCGCGCCAAGCACGCCGCCGCGGCG
GTCGCCGGTTCCTCGGTGATCACCCGCGTCATCGGCATGCCGGCCGACCTGGACGAGGACGATCTGGAGTCGCAGGTCGA
ACTGGAAGCGGCCAATTACGTGCCGTATCCGATCGATGAGGTCAACCACGATTTCGAAGTGCTCGGCCCGATGCCGGGCA
GCCCGGACATGGTCCAGGTGCTGCTGGCGGCCTCGCGCTCGGAGAACGTGGAGGTGCGCGCCTCGGCGCTGGAGCTCGGC
GGCCTGACCCCGAAGGTCATGGACGTGGAGGCCTTCGCGATCGAGAACGCGTTCGCGCTGCTCGCCGACACCCTCAGCAG
CCCGCGCGACGGCCTGGTCGCCCTGGTCGACTCCGGCGCGACCATGACCACGCTCAACGTGCTGCGCAACGGCCGCAGCC
TCTATCACCGCGAGCAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGTTGCCGGAAAGCTACCAGGCCGAGGTGCTGGAGCCGTTCAAGGAAGCCATGGT
CCAGCAGGTCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGCGAGTTCAACCGGGTCGACCAGATCGTTCTGGCCGGCG
GCGGCGCCTCGATCCCGCGCATCGCCGAAATGGTGGAAGAACAGCTCGGCATCCCGACCACGGTCGCCAACCCGCTCGCC
CACATGACCCTGGGGCCGCGCGTGCAGGCGCACGCACTGGCGCAGGACGCGCCCGCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  comM Acinetobacter nosocomialis M2

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

44.193

100

0.443

Multiple sequence alignment