Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   H7R48_RS13515 Genome accession   NZ_AP022298
Coordinates   2836783..2837793 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain BEC1-S17-ESBL-09     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2831783..2842793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R48_RS13495 (BE1S17E09_26350) mepM 2832745..2834067 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  H7R48_RS13500 (BE1S17E09_26360) znuA 2834083..2835015 (-) 933 WP_024180309.1 zinc ABC transporter substrate-binding protein ZnuA -
  H7R48_RS13505 (BE1S17E09_26370) znuC 2835094..2835849 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  H7R48_RS13510 (BE1S17E09_26380) znuB 2835846..2836631 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  H7R48_RS13515 (BE1S17E09_26390) ruvB 2836783..2837793 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  H7R48_RS13520 (BE1S17E09_26400) ruvA 2837802..2838413 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  H7R48_RS13525 yobI 2838552..2838617 (-) 66 WP_010723105.1 stress response small protein YobI -
  H7R48_RS13530 (BE1S17E09_26410) yebB 2838688..2839290 (+) 603 WP_001024914.1 YebB family permuted papain-like enzyme -
  H7R48_RS13535 (BE1S17E09_26420) ruvC 2839292..2839813 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  H7R48_RS13540 (BE1S17E09_26430) yebC 2839848..2840588 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  H7R48_RS13545 (BE1S17E09_26440) nudB 2840617..2841069 (-) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=67439 H7R48_RS13515 WP_000568522.1 2836783..2837793(-) (ruvB) [Escherichia coli strain BEC1-S17-ESBL-09]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=67439 H7R48_RS13515 WP_000568522.1 2836783..2837793(-) (ruvB) [Escherichia coli strain BEC1-S17-ESBL-09]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGTTTAGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGTACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGCCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment