Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MTP21_RS13135 Genome accession   NZ_CP094880
Coordinates   2876303..2876896 (-) Length   197 a.a.
NCBI ID   WP_020334615.1    Uniprot ID   A0AAN1CWF1
Organism   Vibrio natriegens strain WPAGA4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2871303..2881896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP21_RS13115 aroB 2872177..2873277 (-) 1101 WP_014233146.1 3-dehydroquinate synthase -
  MTP21_RS13120 aroK 2873309..2873827 (-) 519 WP_014233147.1 shikimate kinase AroK -
  MTP21_RS13125 pilQ 2874016..2875755 (-) 1740 WP_243975170.1 type IV pilus secretin PilQ family protein Machinery gene
  MTP21_RS13130 pilP 2875798..2876313 (-) 516 WP_243975173.1 pilus assembly protein PilP Machinery gene
  MTP21_RS13135 pilO 2876303..2876896 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  MTP21_RS13140 pilN 2876889..2877464 (-) 576 WP_243975175.1 PilN domain-containing protein Machinery gene
  MTP21_RS13145 pilM 2877448..2878464 (-) 1017 WP_243975186.1 type IV pilus assembly protein PilM Machinery gene
  MTP21_RS13150 - 2878608..2881175 (+) 2568 WP_243975187.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23102.59 Da        Isoelectric Point: 4.7130

>NTDB_id=673904 MTP21_RS13135 WP_020334615.1 2876303..2876896(-) (pilO) [Vibrio natriegens strain WPAGA4]
MVNWQDLEMDEIAEWPLIPQLLVISLLILLLQGFGYWLYLVPKEERLNDLIQQEETLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSDHFLYQLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRAHTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=673904 MTP21_RS13135 WP_020334615.1 2876303..2876896(-) (pilO) [Vibrio natriegens strain WPAGA4]
ATGGTTAATTGGCAAGATCTGGAAATGGATGAGATTGCTGAGTGGCCTTTAATCCCTCAGTTGCTAGTCATTAGTTTGTT
GATCCTGTTGCTCCAAGGTTTCGGCTATTGGCTGTACTTGGTGCCTAAGGAAGAGCGATTAAATGACCTCATCCAGCAGG
AAGAAACTCTCAAAGTTGCGTTACGAATTAAAGCCAACAAGGTTGCCGCATTACCGCAGCTCCAATCTCAGTTAGATGAA
TTGACTACACGTTACGAGTTGTTGTTGCAGCAATTACCCGCCCAAAAAGAGTTAGCCACGATGCTGGCTTCCGTCAATGA
GCTCGGCATAGACAATAAACTGACCTTCACGCGAATTGACTGGGGAGAAAAGCAGTCTGATCACTTTTTGTATCAGCTGC
CACTTAACATCGAGTTAACCGGTGATTTTCATGACATCGGCCGTTTCTCACAAGCGATTGCAACTTTACCACGCATCATC
ACTTTTCAAGATGTGACTTGGCAACGCGTCAGTCAGGAGAGTGAAACGCTGCACTTCCGGGTAAGAGCACATACCTATCA
GTTCAAGCCAGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.947

96.447

0.858
  pilO Vibrio cholerae strain A1552

70.558

100

0.706