Detailed information NTG0673857

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MT343_RS14030
• Genome accession: NZ_CP094879
• Coordinates: 3130306..3131481 (+)
• Length: 391 a.a.
• NCBI ID: WP_145465933.1
• Uniprot ID: A0A558NS28
• Organism: Thalassolituus sp. C2-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3125306..3136481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MT343_RS14005	-	3125763..3126074 (+)	312	WP_145465928.1	hypothetical protein	-
MT343_RS14010	-	3126086..3127096 (-)	1011	WP_145465929.1	hypothetical protein	-
MT343_RS14015	-	3127221..3127697 (-)	477	WP_145465930.1	hypothetical protein	-
MT343_RS14020	-	3127829..3128812 (-)	984	WP_145465931.1	hypothetical protein	-
MT343_RS14025	-	3129083..3129895 (+)	813	WP_145465932.1	hypothetical protein	-
MT343_RS14030	pilU	3130306..3131481 (+)	1176	WP_145465933.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MT343_RS14035	-	3131636..3132031 (+)	396	WP_145465934.1	glutaredoxin family protein	-
MT343_RS14040	-	3132252..3132797 (-)	546	WP_145465935.1	carboxymuconolactone decarboxylase family protein	-
MT343_RS14045	-	3132923..3133834 (+)	912	WP_145465936.1	LysR family transcriptional regulator	-
MT343_RS14050	-	3134057..3134272 (-)	216	WP_145465937.1	hypothetical protein	-
MT343_RS14055	-	3134423..3134845 (-)	423	WP_145465938.1	hypothetical protein	-
MT343_RS14060	-	3134972..3135424 (-)	453	WP_145465939.1	hypothetical protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43014.26 Da
• Isoelectric Point: 7.0821

>NTDB_id=673857 MT343_RS14030 WP_145465933.1 3130306..3131481(+) (pilU) [Thalassolituus sp. C2-1]
MSIENWLHLLAKHDGSDLYLSKGAPPCAKFQGQLKPISRDVLGPGDIEKIAYSIMSEAQRQEFEEEMELNMAMSNSSGRF
RINIFKQQHEVSLVARNINTEIPDIAQLNLPPVLKDVITAKRGLILFVGGTGSGKSTSLAALIDHRNATTYGHIITIEDP
IEFVHKHKKSVINQREVGTDTKSYAAALKNTLRQAPDVILIGEIRDAETMEHALAFAETGHLAISTLHANNANQAFERIV
NMFPEERRNQILLGLGQNVRAIISQRLIPTVDGKRAAAVEVLLGNKTVEDLIIKGDFPSIKEIMNKSENLGMQTFDGALY
HLYKQGKITLEQALKNADSENNLRLRIKLGDNKPESKPAAIKQTAPKAAAPAASGLELSLMDDGEKKEAYA

Nucleotide

• Length: 1176 bp

>NTDB_id=673857 MT343_RS14030 WP_145465933.1 3130306..3131481(+) (pilU) [Thalassolituus sp. C2-1]
ATGAGCATCGAAAACTGGCTTCACCTTCTGGCAAAACACGATGGCTCTGACCTCTACCTGAGCAAAGGTGCTCCACCTTG
CGCGAAATTTCAGGGACAGTTAAAACCCATCAGCCGTGACGTGCTGGGGCCAGGCGACATTGAAAAAATTGCCTATTCCA
TTATGTCGGAGGCACAACGGCAGGAATTTGAAGAAGAAATGGAACTGAATATGGCCATGTCCAATTCATCCGGCCGCTTC
CGTATTAACATATTCAAACAGCAGCACGAAGTGTCACTGGTGGCACGCAATATCAATACCGAAATTCCCGATATTGCCCA
GCTGAATCTGCCACCGGTTCTGAAAGACGTCATTACTGCCAAGCGTGGTTTAATTCTGTTTGTTGGCGGTACCGGTTCTG
GTAAATCCACTTCGCTGGCGGCACTGATTGATCACCGAAATGCCACCACTTACGGCCATATTATTACCATCGAAGATCCG
ATTGAATTTGTGCACAAGCACAAAAAGAGCGTGATTAACCAGCGCGAAGTTGGCACCGACACCAAGAGCTATGCCGCAGC
ATTAAAAAATACCCTGCGTCAGGCACCGGATGTCATTCTGATTGGTGAAATCCGCGACGCCGAAACCATGGAACATGCCC
TCGCCTTTGCCGAAACCGGTCATCTGGCCATTTCCACTCTGCATGCGAACAATGCCAACCAGGCCTTTGAGCGTATTGTG
AATATGTTTCCGGAAGAGCGCCGCAATCAGATTTTATTGGGCCTTGGCCAGAATGTACGCGCGATTATTTCTCAGCGTCT
TATTCCCACTGTTGATGGTAAACGCGCTGCCGCCGTGGAAGTACTGTTGGGTAATAAAACCGTAGAAGATCTGATTATTA
AAGGCGATTTCCCATCCATCAAAGAGATTATGAACAAGTCCGAAAACCTCGGCATGCAAACCTTTGATGGCGCACTCTAC
CATCTGTATAAACAGGGCAAGATTACCCTTGAACAGGCACTGAAAAACGCCGACTCAGAAAACAACCTGCGCCTGCGTAT
TAAACTGGGAGACAACAAACCAGAATCCAAGCCAGCAGCAATAAAACAAACAGCCCCAAAAGCCGCAGCTCCTGCAGCAT
CCGGGCTGGAACTCAGCCTGATGGATGACGGTGAGAAGAAAGAAGCGTACGCCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (63-268)

3D structure

• AlphaFold DB: A0A558NS28

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	50.909	98.465	0.501
pilU	Acinetobacter baylyi ADP1	53.203	91.816	0.488
pilU	Vibrio cholerae strain A1552	48.711	89.258	0.435
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.838	86.701	0.389