Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   MUG61_RS23680 Genome accession   NZ_CP094851
Coordinates   5140250..5141587 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain R20-14     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5135250..5146587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUG61_RS23655 (MUG61_23650) pgeF 5135393..5136121 (-) 729 WP_003123393.1 peptidoglycan editing factor PgeF -
  MUG61_RS23660 (MUG61_23655) rluD 5136118..5137080 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  MUG61_RS23665 (MUG61_23660) - 5137226..5138251 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  MUG61_RS23670 (MUG61_23665) - 5138408..5138653 (+) 246 WP_003102591.1 PP0621 family protein -
  MUG61_RS23675 (MUG61_23670) pilS 5138643..5140235 (+) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  MUG61_RS23680 (MUG61_23675) pilR 5140250..5141587 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  MUG61_RS23685 (MUG61_23680) thiO 5141633..5142727 (-) 1095 WP_012614545.1 glycine oxidase ThiO -
  MUG61_RS23690 (MUG61_23685) - 5142862..5143371 (+) 510 WP_003161431.1 Tfp pilus assembly protein FimT/FimU -
  MUG61_RS23695 (MUG61_23690) - 5143477..5143917 (+) 441 WP_254856905.1 Tfp pilus assembly protein FimT/FimU -
  MUG61_RS23700 (MUG61_23695) pilV 5143973..5144530 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  MUG61_RS23705 (MUG61_23700) pilW 5144527..5145351 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  MUG61_RS23710 (MUG61_23705) pilX 5145348..5145935 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=673607 MUG61_RS23680 WP_003110427.1 5140250..5141587(+) (pilR) [Pseudomonas aeruginosa strain R20-14]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=673607 MUG61_RS23680 WP_003110427.1 5140250..5141587(+) (pilR) [Pseudomonas aeruginosa strain R20-14]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGCCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGTGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCAGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384