Detailed information NTG0673584

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MUG61_RS02025
• Genome accession: NZ_CP094851
• Coordinates: 436912..438060 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain R20-14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431912..443060

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUG61_RS02000 (MUG61_02000)	-	433063..433359 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MUG61_RS02005 (MUG61_02005)	-	433356..433949 (-)	594	WP_003084544.1	YggT family protein	-
MUG61_RS02010 (MUG61_02010)	proC	433960..434781 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MUG61_RS02015 (MUG61_02015)	-	434793..435485 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MUG61_RS02020 (MUG61_02020)	pilT	435700..436734 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MUG61_RS02025 (MUG61_02025)	pilU	436912..438060 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MUG61_RS02030 (MUG61_02030)	czcD	438068..438967 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MUG61_RS02035 (MUG61_02035)	-	439121..439525 (+)	405	WP_003084560.1	NINE protein	-
MUG61_RS02040 (MUG61_02040)	-	439768..441141 (+)	1374	WP_058180676.1	pyridoxal-phosphate dependent enzyme	-
MUG61_RS02045 (MUG61_02045)	-	441138..442322 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=673584 MUG61_RS02025 WP_003100959.1 436912..438060(+) (pilU) [Pseudomonas aeruginosa strain R20-14]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=673584 MUG61_RS02025 WP_003100959.1 436912..438060(+) (pilU) [Pseudomonas aeruginosa strain R20-14]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382