Detailed information NTG0670605

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MTX71_RS12475
• Genome accession: NZ_CP094677
• Coordinates: 2628246..2629394 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Pa150
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2623246..2634394

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTX71_RS12455 (MTX71_12425)	-	2623984..2625168 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
MTX71_RS12460 (MTX71_12430)	-	2625165..2626538 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MTX71_RS12465 (MTX71_12435)	-	2626781..2627185 (-)	405	WP_003084560.1	NINE protein	-
MTX71_RS12470 (MTX71_12440)	czcD	2627339..2628238 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MTX71_RS12475 (MTX71_12445)	pilU	2628246..2629394 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MTX71_RS12480 (MTX71_12450)	pilT	2629572..2630606 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MTX71_RS12485 (MTX71_12455)	-	2630821..2631513 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MTX71_RS12490 (MTX71_12460)	proC	2631525..2632346 (+)	822	WP_105579182.1	pyrroline-5-carboxylate reductase	-
MTX71_RS12495 (MTX71_12465)	-	2632357..2632950 (+)	594	WP_003084544.1	YggT family protein	-
MTX71_RS12500 (MTX71_12470)	-	2632947..2633243 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=670605 MTX71_RS12475 WP_003100959.1 2628246..2629394(-) (pilU) [Pseudomonas aeruginosa strain Pa150]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=670605 MTX71_RS12475 WP_003100959.1 2628246..2629394(-) (pilU) [Pseudomonas aeruginosa strain Pa150]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382