Detailed information NTG0067022

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PA8380_RS02015
• Genome accession: NZ_AP014839
• Coordinates: 433091..434239 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 8380
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 428091..439239

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA8380_RS01990	-	429242..429538 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PA8380_RS01995 (PA8380_04070)	-	429535..430128 (-)	594	WP_003084544.1	YggT family protein	-
PA8380_RS02000 (PA8380_04080)	proC	430139..430960 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PA8380_RS02005 (PA8380_04090)	-	430972..431664 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PA8380_RS02010 (PA8380_04100)	pilT	431879..432913 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PA8380_RS02015 (PA8380_04110)	pilU	433091..434239 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PA8380_RS02020 (PA8380_04120)	czcD	434247..435146 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PA8380_RS02025 (PA8380_04130)	-	435300..435704 (+)	405	WP_003084560.1	NINE protein	-
PA8380_RS02030 (PA8380_04140)	-	435947..437320 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PA8380_RS02035 (PA8380_04150)	-	437317..438501 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=67022 PA8380_RS02015 WP_003110016.1 433091..434239(+) (pilU) [Pseudomonas aeruginosa strain 8380]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=67022 PA8380_RS02015 WP_003110016.1 433091..434239(+) (pilU) [Pseudomonas aeruginosa strain 8380]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382