Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTP24_RS13530 Genome accession   NZ_CP094441
Coordinates   2921343..2922473 (-) Length   376 a.a.
NCBI ID   WP_138529105.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. APM04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2916343..2927473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP24_RS13500 (MTP24_13500) - 2916670..2917563 (+) 894 WP_138529104.1 hypothetical protein -
  MTP24_RS13505 (MTP24_13505) - 2917637..2918113 (+) 477 WP_006793185.1 hypothetical protein -
  MTP24_RS13510 (MTP24_13510) rsmE 2918202..2918936 (+) 735 WP_006793186.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MTP24_RS13515 (MTP24_13515) gshB 2919013..2919963 (+) 951 WP_008132396.1 glutathione synthase -
  MTP24_RS13520 (MTP24_13520) - 2920134..2920691 (+) 558 WP_008132397.1 YqgE/AlgH family protein -
  MTP24_RS13525 (MTP24_13525) ruvX 2920820..2921269 (+) 450 WP_006793189.1 Holliday junction resolvase RuvX -
  MTP24_RS13530 (MTP24_13530) pilU 2921343..2922473 (-) 1131 WP_138529105.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTP24_RS13535 (MTP24_13535) pilT 2922483..2923523 (-) 1041 WP_006793191.1 type IV pilus twitching motility protein PilT Machinery gene
  MTP24_RS13540 (MTP24_13540) - 2923526..2924239 (+) 714 WP_138529107.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTP24_RS13545 (MTP24_13545) proC 2924257..2925078 (+) 822 WP_006793193.1 pyrroline-5-carboxylate reductase -
  MTP24_RS13550 (MTP24_13550) - 2925106..2925639 (+) 534 WP_006793194.1 YggT family protein -
  MTP24_RS13555 (MTP24_13555) - 2925699..2926145 (+) 447 WP_008132403.1 DUF4426 domain-containing protein -
  MTP24_RS13560 (MTP24_13560) - 2926150..2926749 (+) 600 WP_243531657.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41774.62 Da        Isoelectric Point: 5.4536

>NTDB_id=669468 MTP24_RS13530 WP_138529105.1 2921343..2922473(-) (pilU) [Pseudoalteromonas sp. APM04]
MTLSLNRYLITMIEKKGSDLFVSSRLPVSAKINGELIQLDDDKLTDEESLQLVESAMSEKQKAEFHATKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDISDLGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEILLNSPMVAELIKNGDIGGIKEAMAKSNDMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGMGDDDN

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=669468 MTP24_RS13530 WP_138529105.1 2921343..2922473(-) (pilU) [Pseudoalteromonas sp. APM04]
ATGACTTTATCTTTAAACCGTTACTTAATCACTATGATTGAGAAAAAAGGCTCTGATTTATTTGTATCTAGCCGTTTACC
TGTTAGTGCAAAAATAAACGGTGAACTAATTCAATTGGACGATGACAAACTCACTGACGAAGAGTCTTTGCAGTTGGTTG
AGTCTGCGATGAGCGAAAAGCAAAAAGCGGAGTTTCATGCTACTAAAGAATGTAACTTTGCTATTGCAACCGACGAAGGG
CGTTTTCGTATTTCTGCGTTTTGGCAGCGCGATAGAGCAGGTATGGTTATTCGCCGAATTGTGACACAGATCCCTGATAT
CAGTGATTTGGGCCTACCCTCGACACTCACCGACGTCATTATGGCTAAGCGCGGTTTAGTTTTGTTTGTTGGTGGAACAG
GAACCGGTAAGTCGACCTCATTAGCAGCCCTGATTGGCTACAGAAACCGAAACCAACGAGGTCATATTTTAACAATTGAA
GATCCTATTGAGTTTGTTCATGAGCACCGCAAAAGTATTATTACCCAACGCGAAGTAGGGCTCGATACAGATAGTTTTGA
GTCGGCGCTTAAAAGCTCATTACGTCAAGCTCCTGATGTTATTTTAATTGGTGAAATACGCTCGCAAGAAACCATGGAAT
ACGCATTAAGCTTTGCTGAAACAGGGCATTTATGTGTGGCTACACTGCATGCCAACAATGCCAACCAAGCCATTGACCGT
ATTATGCATTTGGTACCAAAAGAAAAGCATGACAAGCTTAAATACGATTTAGCACTTAACTTACGTGCCATTGTAGCTCA
ACAATTAATACCTACCTCAGATGGCGAAGGACGCGTTGCCGCAATAGAGATATTACTTAATTCACCTATGGTGGCAGAGC
TGATTAAAAACGGCGATATTGGCGGCATTAAAGAGGCTATGGCTAAATCTAACGATATGGGCATGCAAACCTTTGACCAA
GCCCTTTTTGAGTTATACAGGCAGCAACGTATTAACTATGCAGATGCATTGCATCATGCTGACTCACCAAATGACTTACG
CTTGATGATTAAACTGCGCAATAACGAACAACAAGGTGCGGGCTTTTTACAAGGTGTCACTATTGATGGAATGGGGGATG
ACGATAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.825

100

0.58
  pilU Acinetobacter baylyi ADP1

55.968

100

0.561
  pilU Vibrio cholerae strain A1552

55.586

97.606

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.84

92.819

0.407

Multiple sequence alignment