Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IEQ11_RS04600 Genome accession   NZ_CP094357
Coordinates   1127959..1129017 (+) Length   352 a.a.
NCBI ID   WP_036108732.1    Uniprot ID   A0A120AGF4
Organism   Lysobacter capsici strain VKM B-2533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1122959..1134017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEQ11_RS04585 (IEQ11_04585) - 1123532..1123690 (-) 159 WP_191821956.1 hypothetical protein -
  IEQ11_RS04590 (IEQ11_04590) - 1123986..1124639 (-) 654 WP_036108820.1 hypothetical protein -
  IEQ11_RS04595 (IEQ11_04595) - 1124889..1127462 (-) 2574 WP_191821955.1 penicillin-binding protein 1A -
  IEQ11_RS04600 (IEQ11_04600) pilM 1127959..1129017 (+) 1059 WP_036108732.1 pilus assembly protein PilM Machinery gene
  IEQ11_RS04605 (IEQ11_04605) - 1129017..1129832 (+) 816 WP_191821954.1 PilN domain-containing protein -
  IEQ11_RS04610 (IEQ11_04610) pilO 1129829..1130539 (+) 711 WP_036108728.1 type 4a pilus biogenesis protein PilO -
  IEQ11_RS04615 (IEQ11_04615) - 1130541..1131092 (+) 552 WP_051547484.1 pilus assembly protein PilP -
  IEQ11_RS04620 (IEQ11_04620) pilQ 1131153..1133219 (+) 2067 WP_228464650.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37818.20 Da        Isoelectric Point: 4.6415

>NTDB_id=669210 IEQ11_RS04600 WP_036108732.1 1127959..1129017(+) (pilM) [Lysobacter capsici strain VKM B-2533]
MGLITKSQPALVGVDISSTAVKLLQLSRVGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRVKHAAAA
VAGSSVITRVIGMPNDLDEDEMESQIELEAANYVPYPIDEVNHDFEILGPMPGASDMVQVLLAASRSENVEVRASALELG
GLTPRVMDVEAFAIENAFALLADTLSGPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGVPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=669210 IEQ11_RS04600 WP_036108732.1 1127959..1129017(+) (pilM) [Lysobacter capsici strain VKM B-2533]
GTGGGACTCATCACAAAAAGCCAGCCGGCCTTGGTCGGCGTGGACATCAGCTCGACCGCGGTAAAGCTGTTGCAGCTCTC
AAGAGTCGGCAACCGCTACCGGGTTGAGCACTATGCTGTCGAACCGCTGCCGCCCAATGCGGTGGTGGAAAAGAACATCG
TCGAGGTCGAAGCGGTGGGCGAGGCGATCAAACGCGCCGTTGCCCGCTCGGGGACCCGCGTGAAGCATGCCGCCGCCGCG
GTCGCCGGATCCTCGGTGATCACCCGGGTCATCGGCATGCCGAACGATCTGGACGAAGACGAGATGGAGTCGCAGATCGA
GCTGGAGGCGGCCAATTACGTGCCGTATCCGATCGACGAGGTGAACCACGACTTCGAGATCCTCGGGCCGATGCCCGGCG
CTTCGGACATGGTCCAGGTGCTGCTCGCGGCCTCGCGATCGGAGAACGTCGAGGTGCGCGCTTCGGCGCTCGAGCTCGGC
GGCCTGACCCCCCGGGTCATGGACGTGGAGGCGTTCGCGATCGAGAACGCGTTCGCGCTGCTCGCCGACACCCTCAGCGG
GCCGCGCGACGGTCTGGTCGCCCTGGTCGATTCCGGCGCGACGATGACCACGCTCAACGTCCTGCGCAACGGCCGCAGCC
TGTATCACCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGTCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGCCAGGGCGGGTTGCCGGAGAGCTACCAGGCCGAAGTGCTGGAGCCGTTCAAGGAAGCGATGGT
CCAGCAGGTCAGTCGCCTGCTGCAGTTCTTCTATGCCGGCAGCGAGTTCAACCGCGTCGATCAGATCGTGCTGGCCGGTG
GCGGCGCCTCGATTCCGCGCATCGCCGAGATGGTCGAGGAGCAACTCGGCGTCCCGACCACCGTGGCCAATCCGCTCGCG
CACATGACCTTGGGCCCGCGCGTCCAGGCGCATGCCCTGGCGCAGGACGCGCCCGCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A120AGF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.284

100

0.503
  comM Acinetobacter nosocomialis M2

50

100

0.5
  comM Acinetobacter baylyi ADP1

49.432

100

0.494
  pilM Legionella pneumophila strain ERS1305867

44.286

99.432

0.44

Multiple sequence alignment