Detailed information NTG0669093

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PM1_RS18145
• Genome accession: NZ_CP094343
• Coordinates: 4115828..4117030 (-)
• Length: 400 a.a.
• NCBI ID: WP_009619518.1
• Uniprot ID: -
• Organism: Pseudomonas sp. M1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4110828..4122030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PM1_RS18120 (MRY70_18120)	-	4111211..4112044 (+)	834	WP_009619538.1	oxidoreductase	-
PM1_RS18125 (MRY70_18125)	-	4112554..4112826 (-)	273	WP_009619536.1	hypothetical protein	-
PM1_RS18130 (MRY70_18130)	-	4113289..4114245 (-)	957	WP_009619524.1	MBL fold metallo-hydrolase	-
PM1_RS18135 (MRY70_18135)	-	4115037..4115375 (+)	339	WP_024128337.1	hypothetical protein	-
PM1_RS18140 (MRY70_18140)	-	4115457..4115606 (-)	150	Protein_3590	glycerophosphodiester phosphodiesterase	-
PM1_RS18145 (MRY70_18145)	pilU	4115828..4117030 (-)	1203	WP_009619518.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PM1_RS18150 (MRY70_18150)	-	4117222..4117500 (+)	279	WP_009619516.1	peptidylprolyl isomerase	-
PM1_RS18155 (MRY70_18155)	-	4117514..4117876 (+)	363	WP_009619514.1	MmcQ/YjbR family DNA-binding protein	-
PM1_RS18160 (MRY70_18160)	-	4117873..4118430 (+)	558	WP_009619499.1	putative glycolipid-binding domain-containing protein	-
PM1_RS18165 (MRY70_18165)	-	4118701..4119900 (+)	1200	WP_009619497.1	MFS transporter	-
PM1_RS18170 (MRY70_18170)	-	4120704..4121984 (+)	1281	WP_009619496.1	OprD family porin	-

Sequence

Protein

• Length: 400 a.a.
• Molecular weight: 43931.09 Da
• Isoelectric Point: 6.0779

>NTDB_id=669093 PM1_RS18145 WP_009619518.1 4115828..4117030(-) (pilU) [Pseudomonas sp. M1]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPAAAQTQAASAQPATPAAPVVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide

• Length: 1203 bp

>NTDB_id=669093 PM1_RS18145 WP_009619518.1 4115828..4117030(-) (pilU) [Pseudomonas sp. M1]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTTTACCTTTCCACCGGCGCGCCGCCTTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAACCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAACAGAAGGCCGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
AGACCTGAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATTACCATCGAGGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCACTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACCCAGGATGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAGATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAACCCGGCCGCGGCCCAGACCCAGGCCGCCAGCGCCCAGCCGGCAACCCCGGCCGCGCCCG
TGGTGGAAGCCTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.695	87.75	0.497
pilU	Acinetobacter baylyi ADP1	53.409	88	0.47
pilU	Vibrio cholerae strain A1552	52.125	88.25	0.46
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.895	95.25	0.38