Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   H7K50_RS10435 Genome accession   NZ_AP022221
Coordinates   2189783..2190793 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain WP8-S17-ESBL-08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2184783..2195793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K50_RS10405 (WP8S17E08_20140) nudB 2186507..2186959 (+) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -
  H7K50_RS10410 (WP8S17E08_20150) yebC 2186988..2187728 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  H7K50_RS10415 (WP8S17E08_20160) ruvC 2187763..2188284 (+) 522 WP_001551239.1 crossover junction endodeoxyribonuclease RuvC -
  H7K50_RS10420 (WP8S17E08_20170) yebB 2188286..2188888 (-) 603 WP_001024929.1 YebB family permuted papain-like enzyme -
  H7K50_RS10425 yobI 2188959..2189024 (+) 66 WP_010723105.1 stress response small protein YobI -
  H7K50_RS10430 (WP8S17E08_20180) ruvA 2189163..2189774 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  H7K50_RS10435 (WP8S17E08_20190) ruvB 2189783..2190793 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  H7K50_RS10440 (WP8S17E08_20200) znuB 2190943..2191728 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  H7K50_RS10445 (WP8S17E08_20210) znuC 2191725..2192480 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  H7K50_RS10450 (WP8S17E08_20220) znuA 2192559..2193491 (+) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  H7K50_RS10455 (WP8S17E08_20230) mepM 2193507..2194829 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=66874 H7K50_RS10435 WP_000568522.1 2189783..2190793(+) (ruvB) [Escherichia coli strain WP8-S17-ESBL-08]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=66874 H7K50_RS10435 WP_000568522.1 2189783..2190793(+) (ruvB) [Escherichia coli strain WP8-S17-ESBL-08]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCCGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment