Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   MP579_RS25065 Genome accession   NZ_CP094240
Coordinates   5446095..5446643 (-) Length   182 a.a.
NCBI ID   WP_003064700.1    Uniprot ID   A0A5S4SNL5
Organism   Comamonas sp. 7D-2evo2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5427433..5446643 5446095..5446643 within 0


Gene organization within MGE regions


Location: 5427433..5446643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MP579_RS24985 (MP579_24985) istB 5427433..5428248 (-) 816 WP_015585970.1 IS21-like element helper ATPase IstB -
  MP579_RS24990 (MP579_24990) istA 5428241..5429749 (-) 1509 WP_024310277.1 IS21-like element ISCsp4 family transposase -
  MP579_RS24995 (MP579_24995) istA 5430344..5431852 (+) 1509 WP_024310277.1 IS21-like element ISCsp4 family transposase -
  MP579_RS25000 (MP579_25000) istB 5431845..5432660 (+) 816 WP_015585970.1 IS21-like element helper ATPase IstB -
  MP579_RS25005 (MP579_25005) - 5432795..5433766 (-) 972 WP_370688535.1 Bug family tripartite tricarboxylate transporter substrate binding protein -
  MP579_RS25010 (MP579_25010) - 5433804..5435411 (-) 1608 WP_242881936.1 thiamine pyrophosphate-binding protein -
  MP579_RS25015 (MP579_25015) - 5435464..5436663 (-) 1200 WP_242881937.1 pyridoxal phosphate-dependent aminotransferase -
  MP579_RS25020 (MP579_25020) - 5436739..5437665 (+) 927 WP_242881938.1 LysR family transcriptional regulator -
  MP579_RS25025 (MP579_25025) - 5437696..5438577 (-) 882 WP_242881939.1 LysR family transcriptional regulator -
  MP579_RS25030 (MP579_25030) argE 5438632..5439798 (-) 1167 WP_242881940.1 acetylornithine deacetylase -
  MP579_RS25035 (MP579_25035) - 5439795..5440313 (-) 519 WP_242881941.1 tripartite tricarboxylate transporter substrate-binding protein -
  MP579_RS25040 (MP579_25040) istA 5440383..5441891 (+) 1509 WP_024310277.1 IS21-like element ISCsp4 family transposase -
  MP579_RS25045 (MP579_25045) istB 5441884..5442699 (+) 816 WP_015585970.1 IS21-like element helper ATPase IstB -
  MP579_RS25050 (MP579_25050) - 5442716..5443099 (-) 384 WP_242881942.1 hypothetical protein -
  MP579_RS25055 (MP579_25055) - 5443092..5444666 (-) 1575 WP_242881943.1 site-specific integrase -
  MP579_RS25060 (MP579_25060) - 5444666..5445967 (-) 1302 WP_003064696.1 tyrosine-type recombinase/integrase -
  MP579_RS25065 (MP579_25065) ssb 5446095..5446643 (-) 549 WP_003064700.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 182 a.a.        Molecular weight: 20057.22 Da        Isoelectric Point: 5.9603

>NTDB_id=668284 MP579_RS25065 WP_003064700.1 5446095..5446643(-) (ssb) [Comamonas sp. 7D-2evo2]
MASVNKVIIVGNLGRDPEMRTFPSGDQVANVTIATTDRWRDKNTGENREATEWHRVVFNGKLAEIVGQYLRKGSQVYVEG
SLRTRKWTDQASGQERYATEIRADAMQMLGSRQGGGQQQGGYGSDDGYGESSYEAPRRQAPAPMQQRPAPAPMQQRPAPA
PMAPPPQRAASGFDDMDDDIPF

Nucleotide


Download         Length: 549 bp        

>NTDB_id=668284 MP579_RS25065 WP_003064700.1 5446095..5446643(-) (ssb) [Comamonas sp. 7D-2evo2]
ATGGCATCCGTCAACAAAGTCATCATCGTCGGCAATCTGGGTCGTGACCCCGAAATGCGCACCTTCCCCAGCGGCGATCA
GGTGGCCAATGTGACCATCGCCACCACAGACCGCTGGCGCGACAAGAACACCGGAGAAAACCGCGAAGCCACCGAGTGGC
ACCGTGTGGTCTTCAACGGCAAACTGGCCGAAATCGTGGGCCAGTACCTGCGCAAAGGCAGCCAGGTCTATGTGGAAGGC
AGCCTGCGCACCCGCAAGTGGACCGACCAGGCCTCCGGCCAGGAACGCTACGCCACCGAAATCCGTGCTGATGCCATGCA
GATGCTGGGCAGCCGCCAGGGTGGTGGTCAGCAGCAAGGCGGCTACGGCAGCGATGATGGCTATGGCGAAAGCAGCTACG
AAGCACCCCGCCGTCAGGCTCCCGCTCCTATGCAGCAGCGCCCCGCACCGGCACCCATGCAGCAGCGTCCTGCTCCTGCA
CCCATGGCTCCTCCTCCCCAGCGCGCAGCTTCGGGGTTTGACGACATGGATGACGACATCCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A5S4SNL5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

53.886

100

0.571

  ssb Glaesserella parasuis strain SC1401

51.562

100

0.544

  ssb Neisseria gonorrhoeae MS11

47.514

99.451

0.473

  ssb Neisseria meningitidis MC58

44.565

100

0.451


Multiple sequence alignment