Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7R13_RS03680 Genome accession   NZ_AP022215
Coordinates   768025..769518 (+) Length   497 a.a.
NCBI ID   WP_000249368.1    Uniprot ID   -
Organism   Escherichia coli strain WP8-S17-ESBL-05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763025..774518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R13_RS03660 (WP8S17E05_07180) pilD 763673..764482 (+) 810 WP_001297673.1 prepilin peptidase PppA Machinery gene
  H7R13_RS03665 (WP8S17E05_07190) gspS2 764548..764958 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  H7R13_RS03670 (WP8S17E05_07200) gspC 764976..765935 (+) 960 WP_000135063.1 type II secretion system protein GspC -
  H7R13_RS03675 (WP8S17E05_07210) gspD 765965..768025 (+) 2061 WP_000498824.1 type II secretion system secretin GspD -
  H7R13_RS03680 (WP8S17E05_07220) pilB 768025..769518 (+) 1494 WP_000249368.1 type II secretion system ATPase GspE Machinery gene
  H7R13_RS03685 (WP8S17E05_07230) gspF 769518..770741 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  H7R13_RS03690 (WP8S17E05_07240) gspG 770758..771213 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  H7R13_RS03695 (WP8S17E05_07250) gspH 771217..771780 (+) 564 WP_023486441.1 type II secretion system minor pseudopilin GspH -
  H7R13_RS03700 (WP8S17E05_07260) gspI 771777..772148 (+) 372 WP_000820104.1 type II secretion system minor pseudopilin GspI -
  H7R13_RS03705 (WP8S17E05_07270) gspJ 772145..772744 (+) 600 WP_001250465.1 type II secretion system minor pseudopilin GspJ -
  H7R13_RS03710 (WP8S17E05_07280) gspK 772747..773724 (+) 978 WP_000633238.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55132.29 Da        Isoelectric Point: 6.1631

>NTDB_id=66769 H7R13_RS03680 WP_000249368.1 768025..769518(+) (pilB) [Escherichia coli strain WP8-S17-ESBL-05]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=66769 H7R13_RS03680 WP_000249368.1 768025..769518(+) (pilB) [Escherichia coli strain WP8-S17-ESBL-05]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGGGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCATCGCTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.332

99.396

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment