Detailed information NTG0066636

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IOMTU433_RS14635
• Genome accession: NZ_AP014649
• Coordinates: 3081918..3083036 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain IOMTU433
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3076918..3088036

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IOMTU433_RS14625 (IOMTU433_2941)	-	3080034..3080726 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
IOMTU433_RS14630 (IOMTU433_2942)	pilT	3080853..3081890 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
IOMTU433_RS14635 (IOMTU433_2943)	pilU	3081918..3083036 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IOMTU433_RS14640 (IOMTU433_2944)	fur	3083106..3083543 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
IOMTU433_RS14645 (IOMTU433_2945)	bamE	3083655..3084053 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
IOMTU433_RS14650 (IOMTU433_2946)	-	3084108..3084431 (-)	324	WP_000442590.1	RnfH family protein	-
IOMTU433_RS14655 (IOMTU433_2947)	-	3084431..3085498 (-)	1068	WP_004748097.1	hypothetical protein	-
IOMTU433_RS14660 (IOMTU433_2948)	-	3085651..3086106 (-)	456	WP_000782976.1	bacteriohemerythrin	-
IOMTU433_RS14665 (IOMTU433_2949)	-	3086359..3087207 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=66636 IOMTU433_RS14635 WP_000347036.1 3081918..3083036(+) (pilU) [Acinetobacter baumannii strain IOMTU433]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=66636 IOMTU433_RS14635 WP_000347036.1 3081918..3083036(+) (pilU) [Acinetobacter baumannii strain IOMTU433]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392