Detailed information NTG0666001

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MO982_RS02000
• Genome accession: NZ_CP093966
• Coordinates: 431431..432579 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ATCC BAA-2114
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 426431..437579

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MO982_RS01975 (MO982_01975)	-	427582..427878 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MO982_RS01980 (MO982_01980)	-	427875..428468 (-)	594	WP_003084544.1	YggT family protein	-
MO982_RS01985 (MO982_01985)	proC	428479..429300 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MO982_RS01990 (MO982_01990)	-	429312..430004 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
MO982_RS01995 (MO982_01995)	pilT	430219..431253 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MO982_RS02000 (MO982_02000)	pilU	431431..432579 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MO982_RS02005 (MO982_02005)	czcD	432587..433486 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MO982_RS02010 (MO982_02010)	-	433640..434044 (+)	405	WP_003084560.1	NINE protein	-
MO982_RS02015 (MO982_02015)	-	434287..435660 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MO982_RS02020 (MO982_02020)	-	435657..436841 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=666001 MO982_RS02000 WP_003100959.1 431431..432579(+) (pilU) [Pseudomonas aeruginosa strain ATCC BAA-2114]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=666001 MO982_RS02000 WP_003100959.1 431431..432579(+) (pilU) [Pseudomonas aeruginosa strain ATCC BAA-2114]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACTAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382