Detailed information NTG0664340

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MON07_RS19530
• Genome accession: NZ_CP093395
• Coordinates: 4173601..4174749 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PA1_NCHU
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4168601..4179749

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MON07_RS19510 (MON07_19510)	-	4169339..4170523 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
MON07_RS19515 (MON07_19515)	-	4170520..4171893 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MON07_RS19520 (MON07_19520)	-	4172136..4172540 (-)	405	WP_003084560.1	NINE protein	-
MON07_RS19525 (MON07_19525)	czcD	4172694..4173593 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MON07_RS19530 (MON07_19530)	pilU	4173601..4174749 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MON07_RS19535 (MON07_19535)	pilT	4174927..4175961 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MON07_RS19540 (MON07_19540)	-	4176176..4176868 (+)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
MON07_RS19545 (MON07_19545)	proC	4176880..4177701 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MON07_RS19550 (MON07_19550)	-	4177712..4178305 (+)	594	WP_003084544.1	YggT family protein	-
MON07_RS19555 (MON07_19555)	-	4178302..4178598 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=664340 MON07_RS19530 WP_003100959.1 4173601..4174749(-) (pilU) [Pseudomonas aeruginosa strain PA1_NCHU]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=664340 MON07_RS19530 WP_003100959.1 4173601..4174749(-) (pilU) [Pseudomonas aeruginosa strain PA1_NCHU]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATTGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382