Detailed information NTG0663912

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MN178_RS02170
• Genome accession: NZ_CP093356
• Coordinates: 483056..484204 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain E125
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 478056..489204

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MN178_RS02145 (MN178_02145)	-	479207..479503 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MN178_RS02150 (MN178_02150)	-	479500..480093 (-)	594	WP_003084544.1	YggT family protein	-
MN178_RS02155 (MN178_02155)	proC	480104..480925 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
MN178_RS02160 (MN178_02160)	-	480937..481629 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MN178_RS02165 (MN178_02165)	pilT	481844..482878 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MN178_RS02170 (MN178_02170)	pilU	483056..484204 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MN178_RS02175 (MN178_02175)	czcD	484212..485111 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MN178_RS02180 (MN178_02180)	-	485265..485669 (+)	405	WP_003084560.1	NINE protein	-
MN178_RS02185 (MN178_02185)	-	485912..487285 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
MN178_RS02190 (MN178_02190)	-	487282..488466 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=663912 MN178_RS02170 WP_003100959.1 483056..484204(+) (pilU) [Pseudomonas aeruginosa strain E125]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=663912 MN178_RS02170 WP_003100959.1 483056..484204(+) (pilU) [Pseudomonas aeruginosa strain E125]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382