Detailed information NTG0663878

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRN73_RS02030
• Genome accession: NZ_CP093355
• Coordinates: 436540..437688 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain E104
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431540..442688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRN73_RS02005 (LRN73_02005)	-	432691..432987 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LRN73_RS02010 (LRN73_02010)	-	432984..433577 (-)	594	WP_003084544.1	YggT family protein	-
LRN73_RS02015 (LRN73_02015)	proC	433588..434409 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LRN73_RS02020 (LRN73_02020)	-	434421..435113 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRN73_RS02025 (LRN73_02025)	pilT	435328..436362 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LRN73_RS02030 (LRN73_02030)	pilU	436540..437688 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LRN73_RS02035 (LRN73_02035)	czcD	437696..438595 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LRN73_RS02040 (LRN73_02040)	-	438749..439153 (+)	405	WP_003084560.1	NINE protein	-
LRN73_RS02045 (LRN73_02045)	-	439396..440769 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LRN73_RS02050 (LRN73_02050)	-	440766..441950 (+)	1185	WP_023114752.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=663878 LRN73_RS02030 WP_003100959.1 436540..437688(+) (pilU) [Pseudomonas aeruginosa strain E104]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=663878 LRN73_RS02030 WP_003100959.1 436540..437688(+) (pilU) [Pseudomonas aeruginosa strain E104]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382