Detailed information NTG0663845

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MN130_RS02190
• Genome accession: NZ_CP093354
• Coordinates: 469619..470767 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain E113
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 464619..475767

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MN130_RS02165 (MN130_02165)	-	465770..466066 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MN130_RS02170 (MN130_02170)	-	466063..466656 (-)	594	WP_003084544.1	YggT family protein	-
MN130_RS02175 (MN130_02175)	proC	466667..467488 (-)	822	WP_031757004.1	pyrroline-5-carboxylate reductase	-
MN130_RS02180 (MN130_02180)	-	467500..468192 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MN130_RS02185 (MN130_02185)	pilT	468407..469441 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MN130_RS02190 (MN130_02190)	pilU	469619..470767 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MN130_RS02195 (MN130_02195)	czcD	470775..471674 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MN130_RS02200 (MN130_02200)	-	471829..472233 (+)	405	WP_003084560.1	NINE protein	-
MN130_RS02205 (MN130_02205)	-	472476..473849 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
MN130_RS02210 (MN130_02210)	-	473846..475030 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=663845 MN130_RS02190 WP_003100959.1 469619..470767(+) (pilU) [Pseudomonas aeruginosa strain E113]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=663845 MN130_RS02190 WP_003100959.1 469619..470767(+) (pilU) [Pseudomonas aeruginosa strain E113]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382