Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   H7R36_RS10495 Genome accession   NZ_AP022161
Coordinates   2231916..2232926 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain WP5-S18-ESBL-08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2226916..2237926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R36_RS10465 (WP5S18E08_20540) nudB 2228640..2229092 (+) 453 WP_077248299.1 dihydroneopterin triphosphate diphosphatase -
  H7R36_RS10470 (WP5S18E08_20550) yebC 2229121..2229861 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  H7R36_RS10475 (WP5S18E08_20560) ruvC 2229896..2230417 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  H7R36_RS10480 (WP5S18E08_20570) yebB 2230419..2231021 (-) 603 WP_022645808.1 YebB family permuted papain-like enzyme -
  H7R36_RS10485 yobI 2231092..2231157 (+) 66 WP_001386853.1 stress response small protein YobI -
  H7R36_RS10490 (WP5S18E08_20580) ruvA 2231296..2231907 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  H7R36_RS10495 (WP5S18E08_20590) ruvB 2231916..2232926 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  H7R36_RS10500 (WP5S18E08_20600) znuB 2233073..2233858 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  H7R36_RS10505 (WP5S18E08_20610) znuC 2233855..2234610 (-) 756 WP_022645806.1 zinc ABC transporter ATP-binding protein ZnuC -
  H7R36_RS10510 (WP5S18E08_20620) znuA 2234689..2235621 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  H7R36_RS10515 (WP5S18E08_20630) mepM 2235637..2236959 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=66375 H7R36_RS10495 WP_000568522.1 2231916..2232926(+) (ruvB) [Escherichia coli strain WP5-S18-ESBL-08]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=66375 H7R36_RS10495 WP_000568522.1 2231916..2232926(+) (ruvB) [Escherichia coli strain WP5-S18-ESBL-08]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAACCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTAGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCACCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment