Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PCAU_RS01430 Genome accession   NZ_AP014623
Coordinates   317771..318823 (+) Length   350 a.a.
NCBI ID   WP_096372864.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312771..323823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCAU_RS01420 (PCAU_0292) - 313723..314991 (+) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -
  PCAU_RS01425 (PCAU_0293) - 315119..317554 (-) 2436 WP_172900888.1 penicillin-binding protein 1A -
  PCAU_RS01430 (PCAU_0294) pilM 317771..318823 (+) 1053 WP_096372864.1 pilus assembly protein PilM Machinery gene
  PCAU_RS01435 (PCAU_0295) - 318823..319389 (+) 567 WP_096372865.1 PilN domain-containing protein -
  PCAU_RS01440 (PCAU_0296) - 319386..320003 (+) 618 WP_096372866.1 type 4a pilus biogenesis protein PilO -
  PCAU_RS01445 (PCAU_0297) - 320000..320527 (+) 528 WP_096372867.1 pilus assembly protein PilP -
  PCAU_RS01450 (PCAU_0298) pilQ 320541..322613 (+) 2073 WP_096372868.1 type IV pilus secretin PilQ Machinery gene
  PCAU_RS01455 (PCAU_0299) aroK 322618..323136 (+) 519 WP_009041824.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37046.52 Da        Isoelectric Point: 4.8956

>NTDB_id=66260 PCAU_RS01430 WP_096372864.1 317771..318823(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAVIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSIEDAG
LAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=66260 PCAU_RS01430 WP_096372864.1 317771..318823(+) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain StFRB508]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACGTCGGTCAAGCTTCTTGAGTT
GAGCCGGGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGTTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCTTATCCGCTGGATGAGGCCGTCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTATTGGCGGCCTGTCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCACGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCATCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAGATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGATAGAAGACGCTGGC
CTGGCGAAAAAGCAGGGCGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.114

100

0.554
  comM Acinetobacter nosocomialis M2

54.83

100

0.551
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment