Detailed information NTG0066218

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AS19_RS11430
• Genome accession: NZ_AP014613
• Coordinates: 2531832..2532896 (-)
• Length: 354 a.a.
• NCBI ID: WP_035459497.1
• Uniprot ID: A0AAD1EZP4
• Organism: Alcanivorax sp. NBRC 101098
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2526832..2537896

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS19_RS11405 (AS19_22870)	aroK	2527322..2527858 (-)	537	WP_035459500.1	shikimate kinase AroK	-
AS19_RS11410 (AS19_22880)	pilQ	2527855..2530026 (-)	2172	WP_011589509.1	type IV pilus secretin PilQ	-
AS19_RS11415 (AS19_22890)	-	2530047..2530574 (-)	528	WP_011589510.1	pilus assembly protein PilP	-
AS19_RS11420 (AS19_22900)	-	2530589..2531266 (-)	678	WP_011589511.1	type 4a pilus biogenesis protein PilO	-
AS19_RS11425 (AS19_22910)	-	2531266..2531832 (-)	567	WP_011589512.1	PilN domain-containing protein	-
AS19_RS11430 (AS19_22920)	pilM	2531832..2532896 (-)	1065	WP_035459497.1	pilus assembly protein PilM	Machinery gene
AS19_RS11435 (AS19_22930)	-	2533096..2535660 (+)	2565	WP_011589514.1	penicillin-binding protein 1A	-
AS19_RS11440 (AS19_22940)	-	2535771..2537045 (-)	1275	WP_011589515.1	malic enzyme-like NAD(P)-binding protein	-
AS19_RS11445 (AS19_22950)	rpmE	2537154..2537372 (-)	219	WP_011589516.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38332.51 Da
• Isoelectric Point: 4.2237

>NTDB_id=66218 AS19_RS11430 WP_035459497.1 2531832..2532896(-) (pilM) [Alcanivorax sp. NBRC 101098]
MLPFLRKKAQTLLGIDISSTAVKLLELSKSGSRYKVESYGVEPLPANAVVEKNISDVEGVGDAIARLVSRTRPGVKAAAV
AVSGSAVITKLIEMDAELDEDALEMQLKSEADQYIPYPMDEVRMDFQVVGPVENNDERVEVLVAASRTENVEMRTDALEI
GGLQPRAVDVEAYAIERAFQLLLPGLPNAEELETVAVFDIGATMTTLNVFHQGRSVYTREQLFGGKQLTEEIQRRYGISM
EEAGLAKKTGELPDDYEGEVLQPFKDAIVQQVNRSLQFFYGSTQFNEVDYILLAGGSSSIADLGDLIQEKVGAGCSVANP
FSDMALANKVNASAIANDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=66218 AS19_RS11430 WP_035459497.1 2531832..2532896(-) (pilM) [Alcanivorax sp. NBRC 101098]
GTGCTGCCTTTCCTCAGAAAAAAGGCCCAGACACTACTGGGGATCGACATCAGCTCCACGGCGGTCAAGCTGTTGGAACT
GTCCAAATCAGGGAGTCGCTACAAGGTCGAGAGCTATGGGGTTGAGCCCCTGCCGGCCAATGCGGTTGTCGAGAAAAATA
TCAGCGACGTGGAAGGAGTGGGTGATGCTATTGCCCGCTTGGTAAGCCGTACGCGTCCCGGGGTAAAAGCGGCGGCGGTG
GCAGTGTCCGGTTCCGCTGTGATTACTAAGCTCATCGAAATGGATGCCGAGCTGGACGAGGATGCATTGGAAATGCAGCT
CAAGTCTGAGGCAGATCAGTACATCCCGTATCCCATGGATGAGGTCCGTATGGACTTTCAGGTTGTGGGGCCGGTTGAGA
ATAACGATGAGCGCGTGGAGGTGTTGGTTGCCGCCTCACGCACAGAGAACGTGGAGATGCGCACCGATGCGTTGGAAATC
GGTGGCCTACAACCGCGAGCGGTGGATGTGGAAGCGTACGCTATTGAGCGGGCTTTTCAGTTGCTTCTGCCCGGTTTGCC
GAATGCGGAGGAGCTAGAAACCGTGGCGGTATTCGATATCGGCGCCACCATGACCACGCTAAACGTATTTCATCAAGGGC
GTAGCGTTTATACCCGCGAGCAGCTTTTCGGGGGCAAGCAGCTCACTGAAGAAATTCAGCGTCGTTACGGTATTTCTATG
GAAGAGGCGGGGTTGGCCAAGAAAACCGGCGAGCTGCCCGATGATTACGAAGGCGAAGTGCTGCAGCCGTTCAAAGATGC
CATTGTCCAGCAGGTGAATCGTTCATTGCAGTTTTTCTATGGCTCCACGCAATTCAATGAAGTGGATTACATCTTGCTGG
CAGGGGGCTCTTCCTCTATCGCAGACTTGGGCGATCTGATTCAGGAAAAGGTGGGCGCGGGTTGTTCAGTGGCTAACCCT
TTCAGTGACATGGCGCTGGCGAACAAGGTGAATGCCTCTGCCATTGCCAATGATGCCCCGGCATTGATGATTGCCTGTGG
CCTGGCATTGAGGAGCTTTGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	54.237	100	0.542
comM	Acinetobacter nosocomialis M2	54.237	100	0.542
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466