Detailed information NTG0661691

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMZ65_RS02100
• Genome accession: NZ_CP093032
• Coordinates: 452060..453208 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain H02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 447060..458208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMZ65_RS02075 (MMZ65_02075)	-	448211..448507 (-)	297	WP_031632852.1	DUF167 domain-containing protein	-
MMZ65_RS02080 (MMZ65_02080)	-	448504..449097 (-)	594	WP_003084544.1	YggT family protein	-
MMZ65_RS02085 (MMZ65_02085)	proC	449108..449929 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MMZ65_RS02090 (MMZ65_02090)	-	449941..450633 (-)	693	WP_004365038.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMZ65_RS02095 (MMZ65_02095)	pilT	450848..451882 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMZ65_RS02100 (MMZ65_02100)	pilU	452060..453208 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
MMZ65_RS02105 (MMZ65_02105)	czcD	453216..454115 (-)	900	WP_004365039.1	CDF family zinc transporter CzcD	-
MMZ65_RS02110 (MMZ65_02110)	-	454270..454674 (+)	405	WP_003084560.1	NINE protein	-
MMZ65_RS02115 (MMZ65_02115)	-	454917..456290 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
MMZ65_RS02120 (MMZ65_02120)	-	456287..457471 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=661691 MMZ65_RS02100 WP_003110016.1 452060..453208(+) (pilU) [Pseudomonas aeruginosa strain H02]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661691 MMZ65_RS02100 WP_003110016.1 452060..453208(+) (pilU) [Pseudomonas aeruginosa strain H02]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTATATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382