Detailed information NTG0661657

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMZ66_RS02265
• Genome accession: NZ_CP093031
• Coordinates: 482533..483681 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 477533..488681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMZ66_RS02240 (MMZ66_02240)	-	478684..478980 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
MMZ66_RS02245 (MMZ66_02245)	-	478977..479570 (-)	594	WP_003084544.1	YggT family protein	-
MMZ66_RS02250 (MMZ66_02250)	proC	479581..480402 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MMZ66_RS02255 (MMZ66_02255)	-	480414..481106 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMZ66_RS02260 (MMZ66_02260)	pilT	481321..482355 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMZ66_RS02265 (MMZ66_02265)	pilU	482533..483681 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MMZ66_RS02270 (MMZ66_02270)	czcD	483689..484588 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
MMZ66_RS02275 (MMZ66_02275)	-	484743..485147 (+)	405	WP_003084560.1	NINE protein	-
MMZ66_RS02280 (MMZ66_02280)	-	485390..486763 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
MMZ66_RS02285 (MMZ66_02285)	-	486760..487944 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=661657 MMZ66_RS02265 WP_003100959.1 482533..483681(+) (pilU) [Pseudomonas aeruginosa strain H03]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661657 MMZ66_RS02265 WP_003100959.1 482533..483681(+) (pilU) [Pseudomonas aeruginosa strain H03]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382