Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MMZ70_RS27360 Genome accession   NZ_CP093023
Coordinates   5917146..5918210 (-) Length   354 a.a.
NCBI ID   WP_023103573.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5912146..5923210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ70_RS27335 (MMZ70_27340) aroK 5912693..5913211 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MMZ70_RS27340 (MMZ70_27345) pilQ 5913223..5915355 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  MMZ70_RS27345 (MMZ70_27350) pilP 5915409..5915933 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  MMZ70_RS27350 (MMZ70_27355) pilO 5915930..5916553 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  MMZ70_RS27355 (MMZ70_27360) pilN 5916550..5917146 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  MMZ70_RS27360 (MMZ70_27365) pilM 5917146..5918210 (-) 1065 WP_023103573.1 type IV pilus assembly protein PilM Machinery gene
  MMZ70_RS27365 (MMZ70_27370) - 5918395..5920866 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -
  MMZ70_RS27370 (MMZ70_27375) - 5920971..5922239 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37964.43 Da        Isoelectric Point: 4.5399

>NTDB_id=661582 MMZ70_RS27360 WP_023103573.1 5917146..5918210(-) (pilM) [Pseudomonas aeruginosa strain H07]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPEHVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=661582 MMZ70_RS27360 WP_023103573.1 5917146..5918210(-) (pilM) [Pseudomonas aeruginosa strain H07]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCACGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACTGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466