Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MMZ71_RS29530 Genome accession   NZ_CP093022
Coordinates   6263700..6264764 (-) Length   354 a.a.
NCBI ID   WP_058154770.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6258700..6269764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ71_RS29505 (MMZ71_29510) aroK 6259235..6259753 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MMZ71_RS29510 (MMZ71_29515) pilQ 6259765..6261909 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  MMZ71_RS29515 (MMZ71_29520) pilP 6261963..6262487 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  MMZ71_RS29520 (MMZ71_29525) pilO 6262484..6263107 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  MMZ71_RS29525 (MMZ71_29530) pilN 6263104..6263700 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  MMZ71_RS29530 (MMZ71_29535) pilM 6263700..6264764 (-) 1065 WP_058154770.1 type IV pilus assembly protein PilM Machinery gene
  MMZ71_RS29535 (MMZ71_29540) - 6264949..6267420 (+) 2472 WP_058154773.1 penicillin-binding protein 1A -
  MMZ71_RS29540 (MMZ71_29545) - 6267516..6268784 (-) 1269 WP_003095842.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37956.45 Da        Isoelectric Point: 4.5405

>NTDB_id=661548 MMZ71_RS29530 WP_058154770.1 6263700..6264764(-) (pilM) [Pseudomonas aeruginosa strain H08]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELESQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=661548 MMZ71_RS29530 WP_058154770.1 6263700..6264764(-) (pilM) [Pseudomonas aeruginosa strain H08]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAGCCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466