Detailed information NTG0661484

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMZ72_RS02030
• Genome accession: NZ_CP093021
• Coordinates: 439605..440753 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H09
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 434605..445753

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMZ72_RS02005 (MMZ72_02005)	-	435756..436052 (-)	297	WP_121367052.1	DUF167 domain-containing protein	-
MMZ72_RS02010 (MMZ72_02010)	-	436049..436642 (-)	594	WP_003155373.1	YggT family protein	-
MMZ72_RS02015 (MMZ72_02015)	proC	436653..437474 (-)	822	WP_241469354.1	pyrroline-5-carboxylate reductase	-
MMZ72_RS02020 (MMZ72_02020)	-	437486..438178 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMZ72_RS02025 (MMZ72_02025)	pilT	438393..439427 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMZ72_RS02030 (MMZ72_02030)	pilU	439605..440753 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MMZ72_RS02035 (MMZ72_02035)	czcD	440761..441660 (-)	900	WP_024918014.1	CDF family zinc transporter CzcD	-
MMZ72_RS02040 (MMZ72_02040)	-	441814..442218 (+)	405	WP_024918013.1	TM2 domain-containing protein	-
MMZ72_RS02045 (MMZ72_02045)	-	442461..443834 (+)	1374	WP_024918012.1	pyridoxal-phosphate dependent enzyme	-
MMZ72_RS02050 (MMZ72_02050)	-	443831..445015 (+)	1185	WP_024918011.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=661484 MMZ72_RS02030 WP_003100959.1 439605..440753(+) (pilU) [Pseudomonas aeruginosa strain H09]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661484 MMZ72_RS02030 WP_003100959.1 439605..440753(+) (pilU) [Pseudomonas aeruginosa strain H09]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382