Detailed information NTG0661423

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MMZ75_RS05280
• Genome accession: NZ_CP093016
• Coordinates: 1129364..1130512 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain H15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1124364..1135512

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMZ75_RS05260 (MMZ75_05260)	-	1125102..1126286 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
MMZ75_RS05265 (MMZ75_05265)	-	1126283..1127656 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
MMZ75_RS05270 (MMZ75_05270)	-	1127899..1128303 (-)	405	WP_003084560.1	NINE protein	-
MMZ75_RS05275 (MMZ75_05275)	czcD	1128457..1129356 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
MMZ75_RS05280 (MMZ75_05280)	pilU	1129364..1130512 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
MMZ75_RS05285 (MMZ75_05285)	pilT	1130690..1131724 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
MMZ75_RS05290 (MMZ75_05290)	-	1131939..1132631 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
MMZ75_RS05295 (MMZ75_05295)	proC	1132643..1133464 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
MMZ75_RS05300 (MMZ75_05300)	-	1133475..1134068 (+)	594	WP_003084544.1	YggT family protein	-
MMZ75_RS05305 (MMZ75_05305)	-	1134065..1134361 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=661423 MMZ75_RS05280 WP_003100959.1 1129364..1130512(-) (pilU) [Pseudomonas aeruginosa strain H15]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=661423 MMZ75_RS05280 WP_003100959.1 1129364..1130512(-) (pilU) [Pseudomonas aeruginosa strain H15]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382